Citric acid production by Koji fermentation using banana peel as a novel substrate

Alagarsamy Karthikeyan, Nallusamy Sivakumar*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

109 Citations (Scopus)


The growing demand for citric acid and the current need for alternative sources have encouraged biotechnologists to search for novel and economical substrates. Koji fermentation was conducted using the peels of banana (Musa acuminata) as an inexpensive substrate for the production of citric acid using Aspergillus niger. Various crucial parameters that affect citric acid production such as moisture content, temperature, pH, inoculum level and incubation time were quantified. Moisture (70%), 28 °C temperature, an initial pH 3, 108 spores/ml as inoculum and 72 h incubation was found to be suitable for maximum citric acid production by A. niger using banana peel as a substrate.

Original languageEnglish
Pages (from-to)5552-5556
Number of pages5
JournalBioresource Technology
Issue number14
Publication statusPublished - Jul 2010


  • Aspergillus niger
  • Banana peel
  • Citric acid
  • Koji fermentation

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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