TY - JOUR
T1 - Optimization of green silver nanoparticles as nanofungicides for management of rice bakanae disease
AU - Shireen Akhter Jahan, Quazi
AU - Sultana, Ziniya
AU - Ud-Daula, Md Asad
AU - Md. Ashikuzzaman, Ashikuzzaman
AU - Md. Shamim Reja, Shamim Reja
AU - Rahman, Md Mahfuzur
AU - Khaton, Amina
AU - Tang, Md Abul Kashem
AU - Rahman, M. Safiur
AU - Hossain, Md Faruquee
AU - Lee, Seung Ju
AU - Rahman, A. T.M.Mijanur
N1 - © 2024 Published by Elsevier Ltd.
PY - 2024/3/30
Y1 - 2024/3/30
N2 - Rice bakanae, a devastating seed-borne disease caused by Fusarium species requires a more attractive and eco-friendly management strategy. The optimization of plant-mediated silver nanoparticles (AgNPs) as nanofungicides by targeting Fusarium species may be a rational approach. In this study, Azadirachta indica leaf aqueous extract-based AgNPs (AiLAE-AgNPs) were synthesized through the optimization of three reaction parameters: A. indica leaf amount, plant extract-to-AgNO3 ratio (reactant ratio), and incubation time. The optimized green AgNPs were characterized using ultraviolet–visible light (UV–Vis) spectroscopy, field emission scanning electron microscopy (FESEM) with energy dispersive X-ray (EDX) spectroscopy, transmission electron microscopy (TEM), dynamic light scattering (DLS), and powder X-ray diffraction (XRD) techniques. The optimal conditions for producing spherical, unique, and diminutive-sized AgNPs ranging from 4 to 27 nm, with an average size of 15 nm, were 2 g AiLAE at a 1:19 ratio (extract-to-AgNO3) and incubated for 4 h. Fusarium isolates collected from infected soils and identified as F. fujikuroi (40) and F. proliferatum (58 and 65) by PCR were used for seed infestation. The AgNPs exhibited concentration-dependent mycelial growth inhibition with EC50 values ranging from 2.95 to 5.50 μg/mL. The AgNPs displayed exposure time-dependent seed disinfectant potential (complete CFU reduction in F. fujikuroi (40) and F. proliferatum (58) was observed at a concentration of 17.24 μg/mL). The optimized green AgNPs were non-toxic to germinating seeds, and completely cured bakanae under net-house conditions, suggesting their great nano-fungicidal potency for food security and sustainable agriculture.
AB - Rice bakanae, a devastating seed-borne disease caused by Fusarium species requires a more attractive and eco-friendly management strategy. The optimization of plant-mediated silver nanoparticles (AgNPs) as nanofungicides by targeting Fusarium species may be a rational approach. In this study, Azadirachta indica leaf aqueous extract-based AgNPs (AiLAE-AgNPs) were synthesized through the optimization of three reaction parameters: A. indica leaf amount, plant extract-to-AgNO3 ratio (reactant ratio), and incubation time. The optimized green AgNPs were characterized using ultraviolet–visible light (UV–Vis) spectroscopy, field emission scanning electron microscopy (FESEM) with energy dispersive X-ray (EDX) spectroscopy, transmission electron microscopy (TEM), dynamic light scattering (DLS), and powder X-ray diffraction (XRD) techniques. The optimal conditions for producing spherical, unique, and diminutive-sized AgNPs ranging from 4 to 27 nm, with an average size of 15 nm, were 2 g AiLAE at a 1:19 ratio (extract-to-AgNO3) and incubated for 4 h. Fusarium isolates collected from infected soils and identified as F. fujikuroi (40) and F. proliferatum (58 and 65) by PCR were used for seed infestation. The AgNPs exhibited concentration-dependent mycelial growth inhibition with EC50 values ranging from 2.95 to 5.50 μg/mL. The AgNPs displayed exposure time-dependent seed disinfectant potential (complete CFU reduction in F. fujikuroi (40) and F. proliferatum (58) was observed at a concentration of 17.24 μg/mL). The optimized green AgNPs were non-toxic to germinating seeds, and completely cured bakanae under net-house conditions, suggesting their great nano-fungicidal potency for food security and sustainable agriculture.
KW - Fusarium species
KW - Green synthesis
KW - Nanofungicides
KW - Optimization
KW - Rice bakanae
KW - Silver nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85188097578&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85188097578&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/0bf2c4af-ef7a-387c-8799-3e6dea112083/
U2 - 10.1016/j.heliyon.2024.e27579
DO - 10.1016/j.heliyon.2024.e27579
M3 - Article
C2 - 38533066
AN - SCOPUS:85188097578
SN - 2405-8440
VL - 10
JO - Heliyon
JF - Heliyon
IS - 6
M1 - e27579
ER -