TY - JOUR
T1 - A sequential injection method for the determination of piroxicam in pharmaceutical formulations using europium sensitized fluorescence
AU - Al-Kindy, Salma M.Z.
AU - Al-Wishahi, Aisha
AU - Suliman, Fakhr Eldin O.
PY - 2004/12/15
Y1 - 2004/12/15
N2 - A simple, selective and sensitive luminescence method for the assay of piroxicam (PX) in pharmaceutical formulation is developed. The method is based on the luminescence sensitization of europium (Eu3+) by complexation with PX. The signal for PX-EU is monitored at λex=358 nm and λem=615 nm. Optimum conditions for the formation of the complex in methanol were 0.01 M TRIS buffer and 0.2 mM of Eu3+ which allows the determination of 100-2000 ppb of pX in batch method and 100-1000 ppb with limit of detection (LOD) = 23.0 ppb using sequential injection analysis (SIA). The relative standard deviations of the method range between 2 and 3% indicating excellent reproducibility of the method. The proposed method was successfully applied for he assay of PX in pharmaceutical formulations (Feldene capsules and tablets). Average recoveries of 101.0±0.3 and 98.8±2.7% were obtained for capsules in methanol using batch and sequential injection (SI) methods, respectively.
AB - A simple, selective and sensitive luminescence method for the assay of piroxicam (PX) in pharmaceutical formulation is developed. The method is based on the luminescence sensitization of europium (Eu3+) by complexation with PX. The signal for PX-EU is monitored at λex=358 nm and λem=615 nm. Optimum conditions for the formation of the complex in methanol were 0.01 M TRIS buffer and 0.2 mM of Eu3+ which allows the determination of 100-2000 ppb of pX in batch method and 100-1000 ppb with limit of detection (LOD) = 23.0 ppb using sequential injection analysis (SIA). The relative standard deviations of the method range between 2 and 3% indicating excellent reproducibility of the method. The proposed method was successfully applied for he assay of PX in pharmaceutical formulations (Feldene capsules and tablets). Average recoveries of 101.0±0.3 and 98.8±2.7% were obtained for capsules in methanol using batch and sequential injection (SI) methods, respectively.
KW - Europium
KW - Piroxicam
KW - Sensitized fluorescence
KW - Sequential injection
UR - http://www.scopus.com/inward/record.url?scp=7244251958&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=7244251958&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2004.04.014
DO - 10.1016/j.talanta.2004.04.014
M3 - Article
C2 - 18969752
AN - SCOPUS:7244251958
SN - 0039-9140
VL - 64
SP - 1343
EP - 1350
JO - Talanta
JF - Talanta
IS - 5 SPEC. ISS.
ER -