A simple, selective and sensitive luminescence method for the assay of piroxicam (PX) in pharmaceutical formulation is developed. The method is based on the luminescence sensitization of europium (Eu3+) by complexation with PX. The signal for PX-EU is monitored at λex=358 nm and λem=615 nm. Optimum conditions for the formation of the complex in methanol were 0.01 M TRIS buffer and 0.2 mM of Eu3+ which allows the determination of 100-2000 ppb of pX in batch method and 100-1000 ppb with limit of detection (LOD) = 23.0 ppb using sequential injection analysis (SIA). The relative standard deviations of the method range between 2 and 3% indicating excellent reproducibility of the method. The proposed method was successfully applied for he assay of PX in pharmaceutical formulations (Feldene capsules and tablets). Average recoveries of 101.0±0.3 and 98.8±2.7% were obtained for capsules in methanol using batch and sequential injection (SI) methods, respectively.
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