TY - JOUR
T1 - The Stability of Analytes of Ionized Magnesium Concentration and Its Reference Range in Healthy Volunteers
AU - Al-Maqbali, Juhaina Salim
AU - Al Alawi, Abdullah M.
AU - Al-Falahi, Zubaida
AU - Falhammar, Henrik
AU - Al-Zakwani, Ibrahim
AU - Al Za’abi, Mohammed
N1 - Publisher Copyright:
© 2023 by the authors.
PY - 2023/9/15
Y1 - 2023/9/15
N2 - This study aimed to determine the stability of refrigerated analytes of iMg concentration at different time intervals and to establish iMg reference range in a cohort of healthy Omani volunteers (≥18 years). The concentrations of iMg were measured using the direct ion-selective electrode technique. Pearson’s and Lin’s concordance correlation coefficients along with the Bland–Altman plot were used to assess the levels of agreement between iMg concentrations of fresh and refrigerated blood samples at different time intervals. The study included 167 volunteers (51% females) with a median age of 21 (range: 20–25) years. The median, 2.5th, and 97.5th percentiles for fresh iMg reference ranges were 0.55, 0.47, and 0.68 mmol/L, respectively. The overall agreement between the fresh and refrigerated iMg concentrations was poor (rho-c = 0.51; p < 0.001). However, according to Altman’s definition, iMg concentrations of the refrigerated samples for a period of ≤1 h had an excellent correlation with the fresh iMg concentrations (Lin’s rho-c = 0.80), with a small average bias difference of 0.009 (95%CI; −0.025–0.043). A cut-off refrigeration period within ≤1 h at 2–8 °C can be considered an alternate time frame for the gold standard measurement (fresh or within 0.5 h).
AB - This study aimed to determine the stability of refrigerated analytes of iMg concentration at different time intervals and to establish iMg reference range in a cohort of healthy Omani volunteers (≥18 years). The concentrations of iMg were measured using the direct ion-selective electrode technique. Pearson’s and Lin’s concordance correlation coefficients along with the Bland–Altman plot were used to assess the levels of agreement between iMg concentrations of fresh and refrigerated blood samples at different time intervals. The study included 167 volunteers (51% females) with a median age of 21 (range: 20–25) years. The median, 2.5th, and 97.5th percentiles for fresh iMg reference ranges were 0.55, 0.47, and 0.68 mmol/L, respectively. The overall agreement between the fresh and refrigerated iMg concentrations was poor (rho-c = 0.51; p < 0.001). However, according to Altman’s definition, iMg concentrations of the refrigerated samples for a period of ≤1 h had an excellent correlation with the fresh iMg concentrations (Lin’s rho-c = 0.80), with a small average bias difference of 0.009 (95%CI; −0.025–0.043). A cut-off refrigeration period within ≤1 h at 2–8 °C can be considered an alternate time frame for the gold standard measurement (fresh or within 0.5 h).
KW - direct ion selective electrode technique
KW - ionized magnesium
KW - reference range
KW - stability
KW - whole blood
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UR - https://www.mendeley.com/catalogue/da99baee-0482-35d2-a987-844afca1b08b/
U2 - 10.3390/biomedicines11092539
DO - 10.3390/biomedicines11092539
M3 - Article
C2 - 37760980
AN - SCOPUS:85172464359
SN - 2227-9059
VL - 11
SP - 2539
JO - Biomedicines
JF - Biomedicines
IS - 9
M1 - 2539
ER -