Tuberous Sclerosis Complex Axis Controls Renal Extracellular Vesicle Production and Protein Content

Fahad Zadjali, Prashant Kumar, Ying Yao, Daniel Johnson, Aristotelis Astrinidis, Peter Vogel, Kenneth W Gross, John J Bissler

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


The tuberous sclerosis complex (Tsc) proteins regulate the conserved mTORC1 growth regulation pathway. We identified that loss of the Tsc2 gene in mouse inner medullary collecting duct (mIMCD) cells induced a greater than two‐fold increase in extracellular vesicle (EV) production compared to the same cells having an intact Tsc axis. We optimized EV isolation using a wellestablished size exclusion chromatography method to produce high purity EVs. Electron microscopy confirmed the purity and spherical shape of EVs. Both tunable resistive pulse sensing (TRPS) and dynamic light scattering (DLS) demonstrated that the isolated EVs possessed a heterogenous size distribution. Approximately 90% of the EVs were in the 100–250 nm size range, while approximately 10% had a size greater than 250 nm. Western blot analysis using proteins isolated from the EVs revealed the cellular proteins Alix and TSG101, the transmembrane proteins CD63, CD81, and CD9, and the primary cilia Hedgehog signaling‐related protein Arl13b. Proteomic analysis of EVs identified a significant difference between the Tsc2‐intact and Tsc2‐deleted cell that correlated well with the increased production. The EVs may be involved in tissue homeostasis and cause disease by overproduction and altered protein content. The EVs released by renal cyst epithelia in TSC complex may serve as a tool to discover the mechanism of TSC cystogenesis and in developing potential therapeutic strategies.

Original languageUndefined/Unknown
Article number1729
Pages (from-to)1729
Number of pages1
JournalInternational Journal of Molecular Sciences
Issue number5
Publication statusPublished - 2020
Externally publishedYes


  • Extracellular vesicles (EVs)
  • MTORC1
  • Renal cyst
  • TSC complex

ASJC Scopus subject areas

  • Molecular Biology
  • Spectroscopy
  • Catalysis
  • Inorganic Chemistry
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this