Thiol concentration, structural characteristics and gelling properties of bovine heart protein concentrates

M. Shafiur Rahman, Gwang woong Go, Jin Kyu Seo, Khalid Gul, Sung Gil Choi, Han Sul Yang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


Consumer demand for proteins from animal sources has been increasing with economic growth of the populations. Freeze-dried ground bovine heart was treated using supercritical-CO2 (SC–CO2) at different pressures (20, 30 and 40 MPa) and hexane to produce a protein concentrate. Thiol concentration, structural and gelling properties of the control bovine heart and bovine heart protein concentrates (BHPCs) were investigated. SC-CO2 treatment at 20 MPa led to an increased (p < 0.05) thiol (–SH) content (74.08 nmol/mg protein) than those for the control and other BHPCs. Fourier–transform infrared (FT–IR) spectroscopy showed increased secondary structure of α–helix and parallel β–sheet content for SC-CO2–treated bovine heart protein when compared to the control and hexane–treated bovine heart protein. Integrated light scattering in protein suspension showed SC-CO2–treated samples had higher cumulative values (Q3) and density (q3) for the particle size distribution. SC-CO2–treated BHPC had better physicochemical properties without structural deterioration of proteins resulting in increased functional properties such as solubility (31.08 g/100 g bovine heart), viscosity and gelling capacity (least gelation concentration 10 g/100 mL water). Bovine heart proteins may serve as a good source of quality proteins for different food applications.

Original languageEnglish
Pages (from-to)175-181
Number of pages7
Publication statusPublished - Aug 2019


  • Bovine heart protein concentrate
  • Gelling properties
  • Structural characteristics
  • Supercritical-CO
  • Thiol concentration

ASJC Scopus subject areas

  • Food Science


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