Thermal and functional characteristics of defatted bovine heart using supercritical CO2 and organic solvent

M. Shafiur Rahman, Khalid Gul, Han Sul Yang, Jiyeon Chun, William L. Kerr, Sung Gil Choi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


BACKGROUND: Consumer demand for low-fat foods has been increasing as a result of attempts to reduce obesity and chronic diseases. Bovine heart was defatted using supercritical CO2 (SC-CO2) at different pressures together with solvent extraction to produce a protein-based functional ingredient for low-fat food products. Thermal and functional characteristics of control and defatted samples were compared. RESULTS: Supercritical CO2 treatment at high pressure results in more removal of fat, producing a protein-rich defatted bovine heart (DBH). The differential scanning calorimeter (DSC) thermograms and SDS-PAGE bands for SC-CO2-treated DBHs were similar to those of the control sample, indicating high protein stability and better functionality. Hexane-treated DBH showed no major thermal peaks and very diffuse bands in SDS-PAGE, indicating denaturation of proteins during solvent extraction. No denaturation of proteins in SC-CO2-treated DBHs resulted in significantly higher water/oil absorption capacities (3320.00 and 2630.00 g kg−1, respectively), total soluble solids (822.20 and 208.71 g kg−1 at pH 3.5 and 6.5, respectively), foaming capacities (149.37%), and emulsion activity (66.89%) than the hexane-treated DBH. CONCLUSION: Supercritical CO2 treatment of DBH led to higher thermal stability and functional properties than the control and hexane-treated DBH. Defatted bovine heart using SC-CO2 can be a functional ingredient for various low-fat and high-protein food products for health-conscious consumers.

Original languageEnglish
Pages (from-to)816-823
Number of pages8
JournalJournal of the Science of Food and Agriculture
Issue number2
Publication statusPublished - Jan 30 2019
Externally publishedYes


  • bovine heart
  • functional characteristics
  • organic solvent
  • supercritical-CO
  • thermal stability

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Agronomy and Crop Science
  • Nutrition and Dietetics


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