TY - JOUR
T1 - The parasitic trematode Fasciola hepatica exhibits mammalian-type glycolipids as well as Gal(Β1-6)Gal-terminating glycolipids that account for cestode serological cross-reactivity
AU - Wuhrer, Manfred
AU - Grimm, Christiane
AU - Dennis, Roger D.
AU - Idris, Mohamed A.
AU - Geyer, Rudolf
N1 - Funding Information:
We wish to acknowledge the expert technical assistance of Peter Kase, Werner Mink, and Siegfried Kühnhardt in GC and GC-MS analysis. This study was supported by the Deutsche Forschungsgemeinschaft (SFB 535, projects A15 and Z1; GE 386/3). It is in partial fulfilment of the requirements of C. Grimm for the degree of doctor of medicine at Giessen University.
PY - 2004/2
Y1 - 2004/2
N2 - Neutral glycosphingolipids from sheep-derived Fasciola hepatica liver flukes were isolated and characterized both structurally and serologically. After HPLC fractionation, glycolipids were analyzed by linkage analysis, enzymatic cleavage, and MALDI-TOF as well as electrospray ionization mass spectrometry. Obtained results revealed the presence of two types of neutral glycolipids. The first group represented mammalian-type species comprising globo- and isoglobotriaosylceramides (Gal(α1-4)Gal(β1-4)Glc(1-1)ceramide and Gal(α1-3)Gal(β1-4)Glc(1-1)ceramide, respectively) as well as Forssman antigen (GalNAc(α1-3)GalNAc(β1-3/4)Gal(α1-4/3)Gal(β1-4 Glc(1-1)ceramide). Applying Helix pomatia agglutinin, recognizing terminal α-linked GalNAc, to cryosections of adult flukes, the latter glycolipid could be localized to the F. hepatica gut. As Forssman antigen from the parasite and sheep host led to identical MALDI-TOF MS profiles, this glycolipid might be acquired from the definitive host. As a second group, highly antigenic glycolipids were structurally characterized as Gal(β1-6)Gal(β1-4)Glc(1-1)ceramide, Gal(β1-6)Gal(α1-3/4)Gal(β1-4)Glc(1-1)ceramide and Gal(β1-6)Gal(β1-6)Gal(α1-3/4)Gal(β1-4)Glc(1-1 ceramide, the latter two structures of which exhibited both isoglobo- or globo-series core structures. Terminal Gal(β1-6)Gal1- motifs have previously been shown to represent antigenic epitopes of neogala-series glycosphingolipids from tape worms. Using human Echinococcus granulosus infection sera, Gal(β1-6)-Gal-terminating glycolipids could be allocated to the gut in adult liver fluke cryosections. Corresponding neogala-reactive antibodies in F. hepatica infection serum were detected by their binding to E. granulosus and Taenia crassiceps neogalaglycosphingolipids. These antibodies might contribute to the known serological cross-reactivity between F. hepatica and parasitic cestode infections.
AB - Neutral glycosphingolipids from sheep-derived Fasciola hepatica liver flukes were isolated and characterized both structurally and serologically. After HPLC fractionation, glycolipids were analyzed by linkage analysis, enzymatic cleavage, and MALDI-TOF as well as electrospray ionization mass spectrometry. Obtained results revealed the presence of two types of neutral glycolipids. The first group represented mammalian-type species comprising globo- and isoglobotriaosylceramides (Gal(α1-4)Gal(β1-4)Glc(1-1)ceramide and Gal(α1-3)Gal(β1-4)Glc(1-1)ceramide, respectively) as well as Forssman antigen (GalNAc(α1-3)GalNAc(β1-3/4)Gal(α1-4/3)Gal(β1-4 Glc(1-1)ceramide). Applying Helix pomatia agglutinin, recognizing terminal α-linked GalNAc, to cryosections of adult flukes, the latter glycolipid could be localized to the F. hepatica gut. As Forssman antigen from the parasite and sheep host led to identical MALDI-TOF MS profiles, this glycolipid might be acquired from the definitive host. As a second group, highly antigenic glycolipids were structurally characterized as Gal(β1-6)Gal(β1-4)Glc(1-1)ceramide, Gal(β1-6)Gal(α1-3/4)Gal(β1-4)Glc(1-1)ceramide and Gal(β1-6)Gal(β1-6)Gal(α1-3/4)Gal(β1-4)Glc(1-1 ceramide, the latter two structures of which exhibited both isoglobo- or globo-series core structures. Terminal Gal(β1-6)Gal1- motifs have previously been shown to represent antigenic epitopes of neogala-series glycosphingolipids from tape worms. Using human Echinococcus granulosus infection sera, Gal(β1-6)-Gal-terminating glycolipids could be allocated to the gut in adult liver fluke cryosections. Corresponding neogala-reactive antibodies in F. hepatica infection serum were detected by their binding to E. granulosus and Taenia crassiceps neogalaglycosphingolipids. These antibodies might contribute to the known serological cross-reactivity between F. hepatica and parasitic cestode infections.
KW - Forssman antigen
KW - Liver-fluke glycolipids
KW - Neogala-series glycolipids
KW - Oligosaccharide structural analysis
KW - Parasitic trematode
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U2 - 10.1093/glycob/cwh021
DO - 10.1093/glycob/cwh021
M3 - Article
C2 - 14638629
AN - SCOPUS:1342263739
SN - 0959-6658
VL - 14
SP - 115
EP - 126
JO - Glycobiology
JF - Glycobiology
IS - 2
ER -