Supercritical fluids and their applications in biotechnology and related areas

John R. Williams*, Anthony A. Clifford, Salim H.R. Al-Saidi

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

46 Citations (Scopus)


This article serves as an overview, introducing the currently popular area of supercritical fluids (SFs) and their uses in biotechnology and related areas. It covers the fundamentals of supercritical science and moves on to the biotechnological and associated applications of these fluids. Subject areas covered include pure substances as supercritical fluids, the properties of supercritical fluids, organic cosolvents, solubility, and the following applications: extraction, chromatography, reactions, particle production, deposition, and the drying of biological specimens. Within each application, and where possible, the basic principles of the technique are given, as well as a description of the history, instrumentation, methodology, uses, problems encountered, and advantages over the traditional, nonsupercritical methods.

Original languageEnglish
Pages (from-to)263-286
Number of pages24
JournalApplied Biochemistry and Biotechnology - Part B Molecular Biotechnology
Issue number3
Publication statusPublished - Nov 2002


  • Particles from gas-saturated solution
  • Rapid expansion of supercritical solutions
  • Solution enhanced dispersion by supercritical fluids
  • Supercritical antisolvent
  • Supercritical fluid chromatography
  • Supercritical fluid extraction
  • Supercritical fluids

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology


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