Recent Trends and Advancements in CRISPR-Based Tools for Enhancing Resistance against Plant Pathogens

Munazza Ijaz, Fahad Khan, Haitham E.M. Zaki, Muhammad Munem Khan, Khlode S.A. Radwan, Yugen Jiang, Jiahui Qian, Temoor Ahmed, Muhammad Shafiq Shahid, Jinyan Luo*, Bin Li*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review


Targeted genome editing technologies are becoming the most important and widely used genetic tools in studies of phytopathology. The “clustered regularly interspaced short palindromic repeats (CRISPR)” and its accompanying proteins (Cas) have been first identified as a natural system associated with the adaptive immunity of prokaryotes that have been successfully used in various genome-editing techniques because of its flexibility, simplicity, and high efficiency in recent years. In this review, we have provided a general idea about different CRISPR/Cas systems and their uses in phytopathology. This review focuses on the benefits of knock-down technologies for targeting important genes involved in the susceptibility and gaining resistance against viral, bacterial, and fungal pathogens by targeting the negative regulators of defense pathways of hosts in crop plants via different CRISPR/Cas systems. Moreover, the possible strategies to employ CRISPR/Cas system for improving pathogen resistance in plants and studying plant–pathogen interactions have been discussed.

Original languageEnglish
Article number1911
Issue number9
Publication statusPublished - May 2023


  • genome editing
  • phytopathology
  • resistance

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Plant Science

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