Production of biosurfactant and antifungal compound by fermented food isolate Bacillus subtilis 20B

Sanket Joshi, Chirag Bharucha, Anjana J. Desai*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

200 Citations (Scopus)


A biosurfactant producing strain, Bacillus subtilis 20B, was isolated from fermented food in India. The strain also showed inhibition of various fungi in in-vitro experiments on Potato Dextrose Agar medium. It was capable of growth at temperature 55 °C and salts up to 7%. It utilized different sugars, alcohols, hydrocarbons and oil as a carbon source, with preference for sugars. In glucose based minimal medium it produced biosurfactant which reduced surface tension to 29.5 mN/m, interfacial tension to 4.5 mN/m and gave stable emulsion with crude oil and n-hexadecane. The biosurfactant activity was stable at high temperature, a wide range of pH and salt concentrations for five days. Oil displacement experiments using biosurfactant containing broth in sand pack columns with crude oil showed 30.22% recovery. The possible application of organism as biocontrol agent and use of biosurfactant in microbial enhanced oil recovery (MEOR) is discussed.

Original languageEnglish
Pages (from-to)4603-4608
Number of pages6
JournalBioresource Technology
Issue number11
Publication statusPublished - Jul 2008
Externally publishedYes


  • Bacillus subtilis
  • Biosurfactant
  • Interfacial tension
  • MEOR, Biocontrol agent
  • Surface tension

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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