TY - JOUR
T1 - Prevalence of aflatoxin B1 contamination in pre- and post-harvest maize kernels, Food products, Poultry and livestock feeds in Tamil Nadu, India
AU - Vijayasamundeeswari, Ayyathurai
AU - Mohankumar, Munusamy
AU - Karthikeyan, Muthusamy
AU - Vijayanandraj, Selvaraj
AU - Paranidharan, Vaikuntavasen
AU - Velazhahan, Rethinasamy
N1 - Funding Information:
This study was supported by the Indian Council of Agricultural Research, New Delhi
PY - 2009/6/1
Y1 - 2009/6/1
N2 - Aflatoxins, a group of mycotoxins mainly produced by Aspergillus flavus and A. parasiticus, have adverse health effects on humans and livestock that ingest aflatoxin- contaminated food products and feeds. To secure the safety of food and feed, regular monitoring of aflatoxin levels is necessary. In order to understand the magnitude of aflatoxin contamination, a survey was conducted in different agro-ecological zones of Tamil Nadu, India and 242 samples consisting of pre- and post-harvest maize kernels, food products, poultry and livestock feeds were collected from farmers' fields, poultry farms, retail shops and supermarkets and analyzed for aflatoxin B1 (AFB1) contamination by enzyme- linked immunosorbent assay (ELISA) using antiserum raised against aflatoxin B1-Bovine serum albumin (AFB1-BSA). The results indicated that 61.3% of the maize kernel samples were contaminated with AFB1 and the levels of AFB1 in 26% of the pre- and post-harvest maize kernels exceeded 20 μg/kg. The highest level of AFB1 (245 μg/kg) was recorded in post-harvest maize kernel samples. In food products AFB1 was detected only in two samples out of 30 samples tested. Furthermore, the levels ranged from 0.6 to 3.7 μg/kg. In poultry feeds, AFB1 was detected in 30 out of 53 samples and the levels ranged from 0.7 to 31.6 μg/kg. Among the 40 livestock feed samples evaluated 29 samples were contaminated with AFB1 at level ranging from 1.8 to 244.9 μg/kg.
AB - Aflatoxins, a group of mycotoxins mainly produced by Aspergillus flavus and A. parasiticus, have adverse health effects on humans and livestock that ingest aflatoxin- contaminated food products and feeds. To secure the safety of food and feed, regular monitoring of aflatoxin levels is necessary. In order to understand the magnitude of aflatoxin contamination, a survey was conducted in different agro-ecological zones of Tamil Nadu, India and 242 samples consisting of pre- and post-harvest maize kernels, food products, poultry and livestock feeds were collected from farmers' fields, poultry farms, retail shops and supermarkets and analyzed for aflatoxin B1 (AFB1) contamination by enzyme- linked immunosorbent assay (ELISA) using antiserum raised against aflatoxin B1-Bovine serum albumin (AFB1-BSA). The results indicated that 61.3% of the maize kernel samples were contaminated with AFB1 and the levels of AFB1 in 26% of the pre- and post-harvest maize kernels exceeded 20 μg/kg. The highest level of AFB1 (245 μg/kg) was recorded in post-harvest maize kernel samples. In food products AFB1 was detected only in two samples out of 30 samples tested. Furthermore, the levels ranged from 0.6 to 3.7 μg/kg. In poultry feeds, AFB1 was detected in 30 out of 53 samples and the levels ranged from 0.7 to 31.6 μg/kg. Among the 40 livestock feed samples evaluated 29 samples were contaminated with AFB1 at level ranging from 1.8 to 244.9 μg/kg.
KW - Aflatoxin B<sub>1</sub>
KW - Enzyme-linked immunosorbent assay
KW - Feed
KW - Zea mays
UR - http://www.scopus.com/inward/record.url?scp=69149093344&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=69149093344&partnerID=8YFLogxK
U2 - 10.2478/v10045-009-0033-1
DO - 10.2478/v10045-009-0033-1
M3 - Article
AN - SCOPUS:69149093344
SN - 1427-4345
VL - 49
SP - 221
EP - 224
JO - Journal of Plant Protection Research
JF - Journal of Plant Protection Research
IS - 2
ER -