TY - JOUR
T1 - Potential of citrus budlings originating in the Middle East as sources of citrus viroids
AU - Al-Harthi, S. A.
AU - Al-Sadi, A. M.
AU - Al-Saady, A. A.
N1 - Funding Information:
Authors would like to acknowledge Dr. Toni Herbert Dietz and Mr. Ahmed Al-Azizi for providing citrus samples and Issa Al-Mahmooli and Shamsa Al-Hilali for technical help. Thanks are due to Sultan Qaboos University for funding this study through the strategic project “ SR/AGR/CROP/10/01 , potential sources of plant pathogenic fungi and bacteria into farms in Oman”.
PY - 2013/6
Y1 - 2013/6
N2 - A study was conducted to determine the potential of citrus budlings originating in the Middle East as sources of citrus viroids. A total of 101 citrus budlings belonging to 9 citrus cultivars which originated from 5 countries were collected from commercial nurseries in 2010. A multiplex reverse transcription polymerase chain reaction (mRT-PCR) was used for detection of CEVd, CBLVd, HSVd, CDVd and CBCVd viroids from the budlings. Multiplex RT-PCR analysis, followed by sequencing of representative isolates, showed that 29, 28, 54, 68 and 79% of the citrus budlings are positive for CEVd, CBLVd, HSVd, CDVd and CBCVd viroids, respectively. Viroids were detected in citrus budlings originating in Oman, Lebanon, Syria, Egypt and Jordon. Viroids from Oman were found to share 94-100% nucleotide similarity with viroids from other parts of the world. Findings from this study provide evidence for circulation of citrus viroids via budlings originating in the Middle East. Strict quarantine measures and certification programs are required to reduce widespread distribution of citrus viroids via citrus budlings originating in these countries.
AB - A study was conducted to determine the potential of citrus budlings originating in the Middle East as sources of citrus viroids. A total of 101 citrus budlings belonging to 9 citrus cultivars which originated from 5 countries were collected from commercial nurseries in 2010. A multiplex reverse transcription polymerase chain reaction (mRT-PCR) was used for detection of CEVd, CBLVd, HSVd, CDVd and CBCVd viroids from the budlings. Multiplex RT-PCR analysis, followed by sequencing of representative isolates, showed that 29, 28, 54, 68 and 79% of the citrus budlings are positive for CEVd, CBLVd, HSVd, CDVd and CBCVd viroids, respectively. Viroids were detected in citrus budlings originating in Oman, Lebanon, Syria, Egypt and Jordon. Viroids from Oman were found to share 94-100% nucleotide similarity with viroids from other parts of the world. Findings from this study provide evidence for circulation of citrus viroids via budlings originating in the Middle East. Strict quarantine measures and certification programs are required to reduce widespread distribution of citrus viroids via citrus budlings originating in these countries.
KW - Acid lime
KW - Exocortis
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U2 - 10.1016/j.cropro.2013.02.006
DO - 10.1016/j.cropro.2013.02.006
M3 - Article
AN - SCOPUS:84875250514
SN - 0261-2194
VL - 48
SP - 13
EP - 15
JO - Crop Protection
JF - Crop Protection
ER -