TY - JOUR
T1 - Polyhydroxyalkanoate (PHA) Production Using Volatile Fatty Acids Derived from the Anaerobic Digestion of Waste Paper
AU - Al Battashi, Huda
AU - Al-Kindi, Shatha
AU - Gupta, Vijai Kumar
AU - Sivakumar, Nallusamy
N1 - Funding Information:
The authors would like to acknowledge The Research Council (TRC), Oman, for financial support (ORG/EBR/14/003). The authors gratefully acknowledge the Central Analytical and Applied Research Unit (CAARU) at the College of Science and the College of Agriculture and Marine Sciences, Sultan Qaboos University, for giving access to analytical facilities. The authors also acknowledge Dr. Annamalai Neelamegam for his help in this project. V.K.G. would like to acknowledge support from the EU 7th Framework Programme for research, technological development, and demonstration activities under research agreement No. 621364 (TUTIC-Green).
PY - 2020
Y1 - 2020
N2 - Waste paper as a resource for polyhydroxyalkanoate (PHA) production through anaerobic digestion is a low-cost strategy to produce bioplastic. In this study, volatile fatty acids (VFAs) produced from waste paper, one of the significant constituents of municipal solid waste, was utilized as a feedstock for polyhydroxyalkanoate (PHA) production. PHA production from synthetic VFAs by Cupriavidus necator was initially optimized under different VFAs concentrations, VFAs ratios, and nitrogen sources. VFAs concentration of 10 g/L, 5:1:4 ratio of acetic, propionic, and butyric acids (HAc:HPr:HBu) and NaNO3 as nitrogen source were considered the optimum conditions with 56.98% PHA and 0.31 g/g yield. Anaerobic digestion of shredded office paper (OP/S) produced the maximum VFAs (521.50 mg/L) after 15 days of incubation and were utilized for PHA synthesis. Almost 2.24-fold increase in the yield of PHA was achieved with limited nutrient medium compared to nutrient contained medium with a PHA content of 53.50 and 23.88%, respectively. PHA production using anaerobic effluent of waste paper is a promising approach where a series of pretreatment processes, the expensive enzymatic hydrolysis, and detoxification were no longer required, suggesting an environmentally friendly way of biopolymer production.
AB - Waste paper as a resource for polyhydroxyalkanoate (PHA) production through anaerobic digestion is a low-cost strategy to produce bioplastic. In this study, volatile fatty acids (VFAs) produced from waste paper, one of the significant constituents of municipal solid waste, was utilized as a feedstock for polyhydroxyalkanoate (PHA) production. PHA production from synthetic VFAs by Cupriavidus necator was initially optimized under different VFAs concentrations, VFAs ratios, and nitrogen sources. VFAs concentration of 10 g/L, 5:1:4 ratio of acetic, propionic, and butyric acids (HAc:HPr:HBu) and NaNO3 as nitrogen source were considered the optimum conditions with 56.98% PHA and 0.31 g/g yield. Anaerobic digestion of shredded office paper (OP/S) produced the maximum VFAs (521.50 mg/L) after 15 days of incubation and were utilized for PHA synthesis. Almost 2.24-fold increase in the yield of PHA was achieved with limited nutrient medium compared to nutrient contained medium with a PHA content of 53.50 and 23.88%, respectively. PHA production using anaerobic effluent of waste paper is a promising approach where a series of pretreatment processes, the expensive enzymatic hydrolysis, and detoxification were no longer required, suggesting an environmentally friendly way of biopolymer production.
KW - Anaerobic digestion
KW - Polyhydroxyalkanoates
KW - Volatile fatty acids
KW - Waste paper
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U2 - 10.1007/s10924-020-01870-0
DO - 10.1007/s10924-020-01870-0
M3 - Article
AN - SCOPUS:85090221093
SN - 1566-2543
VL - 29
SP - 250
EP - 259
JO - Journal of Polymers and the Environment
JF - Journal of Polymers and the Environment
IS - 1
ER -
