Physio-biochemic and Genetic Changes in Stored Pea (Pisum sativum) Seeds

M. M. Khan*, M. Abbas, F. S. Awan, M. Shahid, M. Ali, S. Ahmad

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Studies were carried out to evaluate the physiological and biochemical changes in pea seeds (cv. Meteor) stored at 5±1°C and ambient temperature 25±2°C. RAPD makers were used to identify any genetic dissimilarity in pea seeds stored up to two years under different storage environments. Maximum germination (98.33%) was recorded in seeds from freshly harvest seeds and minimum was 90% in 2 years old seed stored at 5°C±1. The decline in vigor was rapid in one year seed stored under ordinary condition compared to other storage environment. The α- and β-amylase activity was higher in recent harvest seeds compared to stored seeds. The storage period showed significant effects on α- and β- amylase activity and a decreasing trend was observed as the storage period prolonged. Similarly, peroxidase and catalase activity was also higher in fresh seeds in contrast to α- and β-amylase. Genetic analysis (RAPD) showed 2% dissimilarity in genetic profile amongst the seed lots. However, the seeds stored for longer period regardless the storage environment, have more genetic damage. The physiological, biochemical and genetic changes could be helpful in understanding the mechanism of pea seeds deterioration.

Original languageEnglish
Pages (from-to)951-956
Number of pages6
JournalInternational Journal of Agriculture and Biology
Issue number5
Publication statusPublished - 2013


  • Amylase
  • CAT
  • DNA
  • Germination
  • POD
  • Pisum sativum
  • RAPD
  • Seed ageing
  • Seed storage
  • Vigor

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)


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