Molecular detection of rifampicin resistance in Mycobacterium tuberculosis: An urban study

Salasawati Hussin; Srijit Das; Yew Lok Woo; Farizal Nordin; Farizan Zakil; Hadi Razak; Norhayati Jamaludin; Nordiana Ismail; Noor Hamiza Ghulam; Ainilhayati Nordin; Sharifah Azura Salleh

Molecular detection of rifampicin resistance in Mycobacterium tuberculosis: An urban study

Salasawati Hussin^*, Srijit Das, Yew Lok Woo, Farizal Nordin, Farizan Zakil, Hadi Razak, Norhayati Jamaludin, Nordiana Ismail, Noor Hamiza Ghulam, Ainilhayati Nordin, Sharifah Azura Salleh

^*Corresponding author for this work

Human & Clinical Anatomy

Research output: Contribution to journal › Article › peer-review

Abstract

Objective: The detection of multi-drug resistant tuberculosis (MDR-TB) by broth method requires eight weeks. Molecular technique shortens the duration and it is able to detect the resistant gene. The objective of this study was to determine the feasibility of using molecular technique to detect MDR-TB compared to broth method. Materials and methods: Samples were collected from 726 patients suspected to have tuberculosis throughout the year 2008 in Universiti Kebangsaan Malaysia Medical Centre (UKMMC). Growth on Lowenstein-Jensen media were confirmed as Mycobacterium tuberculosis using BDProbeTec^™ ET Mycobacterium and susceptibility testing was performed using BACTEC ^™ MGIT^™ 960. The 411bp fragment of rpo gene was amplified and sequenced. Results: Fifty of the 726 samples (6.89%) grew Mycobacterium tuberculosis. MDR was detected in four of the 50 (8%) isolates of Mycobacterium tuberculosis. The sensitivity of molecular technique in detecting MDR-TB was 25% with a specificity of 100%. The positive predictive value was 100% whereas negative predictive value was 89.66%. Conclusion: Molecular technique was not an effective screening test for MDR-TB due to the low sensitivity.

Original language	English
Pages (from-to)	63-69
Number of pages	7
Journal	International Medical Journal
Volume	18
Issue number	1
Publication status	Published - Mar 2011

Keywords

Drug resistance
Microbiology
Molecular
Tuberculosis
rpo gene

ASJC Scopus subject areas

Medicine(all)

Cite this

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title = "Molecular detection of rifampicin resistance in Mycobacterium tuberculosis: An urban study",

abstract = "Objective: The detection of multi-drug resistant tuberculosis (MDR-TB) by broth method requires eight weeks. Molecular technique shortens the duration and it is able to detect the resistant gene. The objective of this study was to determine the feasibility of using molecular technique to detect MDR-TB compared to broth method. Materials and methods: Samples were collected from 726 patients suspected to have tuberculosis throughout the year 2008 in Universiti Kebangsaan Malaysia Medical Centre (UKMMC). Growth on Lowenstein-Jensen media were confirmed as Mycobacterium tuberculosis using BDProbeTec{\texttrademark} ET Mycobacterium and susceptibility testing was performed using BACTEC {\texttrademark} MGIT{\texttrademark} 960. The 411bp fragment of rpo gene was amplified and sequenced. Results: Fifty of the 726 samples (6.89%) grew Mycobacterium tuberculosis. MDR was detected in four of the 50 (8%) isolates of Mycobacterium tuberculosis. The sensitivity of molecular technique in detecting MDR-TB was 25% with a specificity of 100%. The positive predictive value was 100% whereas negative predictive value was 89.66%. Conclusion: Molecular technique was not an effective screening test for MDR-TB due to the low sensitivity.",

keywords = "Drug resistance, Microbiology, Molecular, Tuberculosis, rpo gene",

author = "Salasawati Hussin and Srijit Das and Woo, {Yew Lok} and Farizal Nordin and Farizan Zakil and Hadi Razak and Norhayati Jamaludin and Nordiana Ismail and Ghulam, {Noor Hamiza} and Ainilhayati Nordin and Salleh, {Sharifah Azura}",

year = "2011",

month = mar,

language = "English",

volume = "18",

pages = "63--69",

journal = "International Medical Journal",

issn = "1341-2051",

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TY - JOUR

T1 - Molecular detection of rifampicin resistance in Mycobacterium tuberculosis

T2 - An urban study

AU - Hussin, Salasawati

AU - Das, Srijit

AU - Woo, Yew Lok

AU - Nordin, Farizal

AU - Zakil, Farizan

AU - Razak, Hadi

AU - Jamaludin, Norhayati

AU - Ismail, Nordiana

AU - Ghulam, Noor Hamiza

AU - Nordin, Ainilhayati

AU - Salleh, Sharifah Azura

PY - 2011/3

Y1 - 2011/3

N2 - Objective: The detection of multi-drug resistant tuberculosis (MDR-TB) by broth method requires eight weeks. Molecular technique shortens the duration and it is able to detect the resistant gene. The objective of this study was to determine the feasibility of using molecular technique to detect MDR-TB compared to broth method. Materials and methods: Samples were collected from 726 patients suspected to have tuberculosis throughout the year 2008 in Universiti Kebangsaan Malaysia Medical Centre (UKMMC). Growth on Lowenstein-Jensen media were confirmed as Mycobacterium tuberculosis using BDProbeTec™ ET Mycobacterium and susceptibility testing was performed using BACTEC ™ MGIT™ 960. The 411bp fragment of rpo gene was amplified and sequenced. Results: Fifty of the 726 samples (6.89%) grew Mycobacterium tuberculosis. MDR was detected in four of the 50 (8%) isolates of Mycobacterium tuberculosis. The sensitivity of molecular technique in detecting MDR-TB was 25% with a specificity of 100%. The positive predictive value was 100% whereas negative predictive value was 89.66%. Conclusion: Molecular technique was not an effective screening test for MDR-TB due to the low sensitivity.

AB - Objective: The detection of multi-drug resistant tuberculosis (MDR-TB) by broth method requires eight weeks. Molecular technique shortens the duration and it is able to detect the resistant gene. The objective of this study was to determine the feasibility of using molecular technique to detect MDR-TB compared to broth method. Materials and methods: Samples were collected from 726 patients suspected to have tuberculosis throughout the year 2008 in Universiti Kebangsaan Malaysia Medical Centre (UKMMC). Growth on Lowenstein-Jensen media were confirmed as Mycobacterium tuberculosis using BDProbeTec™ ET Mycobacterium and susceptibility testing was performed using BACTEC ™ MGIT™ 960. The 411bp fragment of rpo gene was amplified and sequenced. Results: Fifty of the 726 samples (6.89%) grew Mycobacterium tuberculosis. MDR was detected in four of the 50 (8%) isolates of Mycobacterium tuberculosis. The sensitivity of molecular technique in detecting MDR-TB was 25% with a specificity of 100%. The positive predictive value was 100% whereas negative predictive value was 89.66%. Conclusion: Molecular technique was not an effective screening test for MDR-TB due to the low sensitivity.

KW - Drug resistance

KW - Microbiology

KW - Molecular

KW - Tuberculosis

KW - rpo gene

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Molecular detection of rifampicin resistance in Mycobacterium tuberculosis: An urban study

Abstract

Keywords

ASJC Scopus subject areas

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