TY - JOUR
T1 - Loci impacting polymorphic gait in the Tennessee Walking Horse
AU - Staiger, E. A.
AU - Abri, M. A.
AU - Silva, C. A.S.
AU - Brooks, S. A.
N1 - Funding Information:
This work was supported in part by a seed grant from the Cornell Center for Vertebrate Genomics, by the USDA National Institute of Food and Agriculture, Hatch project NYC-127454, and by a grant from the Foundation for the Advancement and Support of the Tennessee Walking Show Horse. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the National Institute of Food and Agriculture (NIFA) or the United States Department of Agriculture (USDA). We appreciate the help of all contributing owners, breeders, and trainers.
Publisher Copyright:
© 2016 American Society of Animal Science. All rights reserved.
PY - 2016/4
Y1 - 2016/4
N2 - Following domestication, man selected the horse primarily for the purpose of transportation rather than consumption; this selective strategy created divergent traits for locomotion. At intermediate speeds, beyond the flat walk, the horse can perform a range of diagonal and lateral 2-beat or 4-beat gait patterns. The Tennessee Walking Horse (TWH) is the only U.S. breed able to perform an even-timed 4-beat gait (the “running-walk”) at intermediate speeds; however, within the breed, there is remaining variation in gait type. To investigate the contribution of genetics to this unique trait, blood or hair samples for DNA and gait information were collected from 129 TWH and genotyping was performed at approximately 60,000 loci using the Illumina Equine SNP70 beadchip at GeneSeek Inc. (Lincoln, NE). Case–control association tests identified suggestive regions for gait type on equine chromosome (ECA) 19 (P-value of 1.50 × 10–5 after 1 million permutations; PLINK version 1.07). Haplotype analysis identified 2 significant haplotypes on ECA19 and ECA11 (P-values of 3.7 × 10–5 and 3.92 × 10–5, respectively). Genes within these suggestive regions play roles in developmental processes and biological regulation, indicating there may be variant differences in the neurobiology and regulation of horses with a polymorphic gait.
AB - Following domestication, man selected the horse primarily for the purpose of transportation rather than consumption; this selective strategy created divergent traits for locomotion. At intermediate speeds, beyond the flat walk, the horse can perform a range of diagonal and lateral 2-beat or 4-beat gait patterns. The Tennessee Walking Horse (TWH) is the only U.S. breed able to perform an even-timed 4-beat gait (the “running-walk”) at intermediate speeds; however, within the breed, there is remaining variation in gait type. To investigate the contribution of genetics to this unique trait, blood or hair samples for DNA and gait information were collected from 129 TWH and genotyping was performed at approximately 60,000 loci using the Illumina Equine SNP70 beadchip at GeneSeek Inc. (Lincoln, NE). Case–control association tests identified suggestive regions for gait type on equine chromosome (ECA) 19 (P-value of 1.50 × 10–5 after 1 million permutations; PLINK version 1.07). Haplotype analysis identified 2 significant haplotypes on ECA19 and ECA11 (P-values of 3.7 × 10–5 and 3.92 × 10–5, respectively). Genes within these suggestive regions play roles in developmental processes and biological regulation, indicating there may be variant differences in the neurobiology and regulation of horses with a polymorphic gait.
KW - Gait
KW - Genome-wide association study
KW - Horse
KW - Running walk
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U2 - 10.2527/jas.2015-9936
DO - 10.2527/jas.2015-9936
M3 - Article
C2 - 27135997
AN - SCOPUS:84971417236
SN - 0021-8812
VL - 94
SP - 1377
EP - 1386
JO - Journal of Animal Science
JF - Journal of Animal Science
IS - 4
ER -