TY - JOUR
T1 - Isolongifolene attenuates rotenone-induced mitochondrial dysfunction, oxidative stress and apoptosis
AU - Balakrishnan, Rengasamy
AU - Elangovan, Namasivayam
AU - Mohankumar, Thangavel
AU - Nataraj, Jagatheesan
AU - Manivasagam, Thamilarasan
AU - Thenmozhi, Arokiasamy Justin
AU - Essa, Musthafa Mohamed
AU - Akbar, Mohammed
AU - Sattar Khan, Mohammed Abdul
N1 - Funding Information:
1Department of Biotechnology, School of Biosciences, Periyar University, Periyar Palgalai nagar, Salem-636011, Tamilnadu, India, 2Department of Biochemistry and Biotechnology, Annamalai University, Annamalai nagar-608 002, Tamilnadu, India, 3Department of Food Science and Nutrition, CAMS, Sultan Qaboos University, Muscat, Oman, 4Ageing and Dementia Research Group, Sultan Qaboos University, Muscat, Oman, 5Food and Brain Research Foundation, Chennai, Tamil Nadu 600094, India, 6NIAA, National Institute of Health, Rockville, MD, USA, 7Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School and Shriners Hospitals for Children, Boston, MA 02114, USA
Publisher Copyright:
© 2018 Frontiers in Bioscience. All Rights Reserved.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - The present study was carried out to investigatetheneuroprotectiveeffectsofisolongifolene (ILF), a tricyclic sesquiterpene of Murraya koenigii, against rotenone-induced mitochondrial dysfunction. Oxidative stress and apoptosis in a cellular model. SH-SY5Y human neuroblastoma cells were divided into four experimental groups (control, rotenone (100 nM), ILF (10 microM) + rotenone (100 nanoM), ILF 10 microM alone treated) based on 3-(4, 5-dimethyl 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The results of the present study showed that the ILF treatment significantly alleviated rotenone-induced cytotoxicity, oxidative stress and mitochondrial dysfunction in SH-SY5Y cells. Moreover, ILF attenuated rotenone induced toxicity by down-regulating Bax, caspases-3, 6, 8 and 9 expression and up-regulating of Bcl-2 expression. Furthermore regulation of p-P13K, p-AKT and p-GSK-3 beta expression by ILF, clearly confirmed its protective effects. Taken together, our results suggested that ILF attenuated rotenone-induced oxidative stress, mitochondrial dysfunction and apoptosis through the regulation of P13K/AKT/GSK-3 beta signaling pathways. However further pre-clinical studies are warranted in rodents to use ILF as a promising therapeutic agent for PD in future.
AB - The present study was carried out to investigatetheneuroprotectiveeffectsofisolongifolene (ILF), a tricyclic sesquiterpene of Murraya koenigii, against rotenone-induced mitochondrial dysfunction. Oxidative stress and apoptosis in a cellular model. SH-SY5Y human neuroblastoma cells were divided into four experimental groups (control, rotenone (100 nM), ILF (10 microM) + rotenone (100 nanoM), ILF 10 microM alone treated) based on 3-(4, 5-dimethyl 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The results of the present study showed that the ILF treatment significantly alleviated rotenone-induced cytotoxicity, oxidative stress and mitochondrial dysfunction in SH-SY5Y cells. Moreover, ILF attenuated rotenone induced toxicity by down-regulating Bax, caspases-3, 6, 8 and 9 expression and up-regulating of Bcl-2 expression. Furthermore regulation of p-P13K, p-AKT and p-GSK-3 beta expression by ILF, clearly confirmed its protective effects. Taken together, our results suggested that ILF attenuated rotenone-induced oxidative stress, mitochondrial dysfunction and apoptosis through the regulation of P13K/AKT/GSK-3 beta signaling pathways. However further pre-clinical studies are warranted in rodents to use ILF as a promising therapeutic agent for PD in future.
KW - Apoptosis
KW - Isolongifolene
KW - Mitochondrial Dysfunction
KW - Oxidative Stress
KW - Rotenone
KW - Signaling Pathway
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U2 - 10.2741/s513
DO - 10.2741/s513
M3 - Article
C2 - 28930531
AN - SCOPUS:85037977952
SN - 1945-0516
VL - 10
SP - 248
EP - 261
JO - Frontiers in Bioscience - Scholar
JF - Frontiers in Bioscience - Scholar
IS - 2
ER -