Improvement of Water Hyacinth Bioconversion by Different Organic and Mineral Acid Pretreatment and the Effect of Post-pretreatment Washing

Marttin Paulraj Gundupalli, Prapakorn Tantayotai, Santi Chuetor, Kraipat Cheenkachorn, Sanket Joshi, Debraj Bhattacharyya, Malinee Sriariyanun*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Water hyacinth is a non-edible plant having a severe impact on aquatic ecosystems through native vegetation displacement and lower dissolved oxygen concentration. High cellulose and low lignin content make water hyacinth a potential source for biofuel production. Water hyacinth was subjected to acid pretreatment using organic acids (citric acid (CA) and oxalic acid (OA)) and mineral acid (hydrochloric acid (HA)) to enhance enzymatic saccharification, and ethanol and biogas production. Under optimized pretreatment condition, the reducing sugars released from enzymatic saccharifications of CA-, OA-, and HA-pretreated samples increased by 2.56-, 1.71-, and 1.62-fold, respectively, than untreated sample. Maximum ethanol yield (8.97 g/L) was observed for OA-pretreated (1.68-fold increase) than untreated water hyacinth, whereas CA-pretreated sample produced the highest biogas yield (3421.5 mL) after anaerobic digestion for 45 days. The increase in the yield of ethanol and biogas for OA and CA is attributed to the changes in the hemicellulose and lignin structure. The change in the structural morphology was observed through FTIR characterization of untreated and treated water hyacinth. In addition, the effect of post-washing after pretreatment on fermentation efficiency was evaluated and the result suggested that CA residues had no negative effect on ethanol production. Pretreatment of water hyacinth using organic acids could benefit the biorefineries through the biofuel production and reduction of wastewater generated from this process.

Original languageEnglish
Pages (from-to)1718-1732
Number of pages15
JournalBioenergy Research
Issue number3
Publication statusPublished - Oct 22 2022


  • Anaerobic digestion
  • Fermentation
  • Mineral acid
  • Organic acid
  • Post-wash
  • Pretreatment

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Agronomy and Crop Science
  • Energy (miscellaneous)

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