Identification by SEM and screening of antibacterial and antioxidant activity of Pteropyrum Scoparium Jaub & Spach Leaves using green solvent extracts

Hakikulla Shah*, Mohammad Sadat Ali, Fatima Al-Malki, Nejib Guizani, Philip Fletcher, Vandita Singh

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


The leaves of Pteropyrum scoparium Jaub & Spach are used by the locals in Oman for treating various stomach infirmities including dyspepsia. No analysis of this species in terms of bioactive composition is reported, however members of this family are reported to contain flavonoids and anthraquinones. This study aimed to explore the bioactive compounds in the Pteropyrum scoparium Jaub & Spach leaves and optimize the bioactive compound extraction process using green solvents such as water, 30 % aqueous glycerol and ethanol. We compared the extraction ability of the solvents by estimate the polyphenols and further evaluated the flavonoids and radical scavenging capacity of the liquid crude extract (LCE) s. We also screened the LCEs for presence of any antibacterial ability on selected ATCC gram positive and gram negative bacterial strains. We established the identification data by recording the botanical characters of the leaves using scanning electron microscopy (SEM) so that the future identification and quality assessment can have strong basis. In extraction process we found the 30 % aqueous glycerol to be most efficient solvent in extraction yield of polyphenols followed by water. However, water LCE was found to be highest in the total flavonoids as well as in radical scavenging capacity. Anti-bacterial screening showed average inhibitory effect on the bacterial growth, interestingly the ethanol LCE showed activity against the gram negative E. coli. Overall as we optimized the extraction process for the leaves of Pteropyrum scoparium Jaub & Spach using green solvents, it is considered safe and offers great potential for further purification and application in future studies.

Original languageEnglish
Pages (from-to)203-208
Number of pages6
JournalOriental Pharmacy and Experimental Medicine
Issue number3
Publication statusPublished - Sept 1 2016


  • Antibacterial activity
  • Antioxidant activity
  • Green solvent
  • Polyphenols
  • Pteropyrum scoparium
  • SEM

ASJC Scopus subject areas

  • Complementary and alternative medicine


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