Fluorescence quenching studies of nitrated polycyclic aromatic hydrocarbons

Salma M.Z. Al-Kindy, James N. Miller

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


The analysis of nitrated polycyclic aromatic hydrocarbons (NPAHs) is of great importance because of the mutagenicity and possible carcinogenic activity of these compounds, which are distributed widely in the environment. Nitro-substituents in aromatic compounds are known to quench fluorescence and NPAHs have no intrinsic fluorescence, but they can be determined using their quenching effects on other fluorophores. The quenching effects of several important NPAHs on 1,2,3,4- tetrahydro-1-naphthol,5,6,7,8-tetrahydro-1-naphthol, 4-(2-hydroxy-4-sulfo-1-naphthylazo)-2-naphthalene carboxylic acid and 7-amino-4-methyl coumarin have been studied. The singlet emission of these fluorophores is efficiently quenched by all the NPAHs, the quenching following the Stern-Volmer relationship. Quenching constants and the limits of detection and linear ranges of the quenchers have been determined in each case: the limits of detection are ca 1 μm.

Original languageEnglish
Pages (from-to)148-152
Number of pages5
Issue number2
Publication statusPublished - 2011


  • Stern -Volmer
  • fluorescence quenching
  • fluorophores
  • nitrated polycyclic aromatic hydrocarbons

ASJC Scopus subject areas

  • Biophysics
  • Chemistry (miscellaneous)


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