First report of a ‘candidatus phytoplasma aurantifolia’-related strain in citrus macrophylla in Oman

Ali M. Al-Subhi; Rashid A. Al-Yahyai; Abdullah M. Al-Sadi

doi:10.5958/2249-4677.2019.00004.5

First report of a ‘candidatus phytoplasma aurantifolia’-related strain in citrus macrophylla in Oman

Ali M. Al-Subhi, Rashid A. Al-Yahyai, Abdullah M. Al-Sadi

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Citrus macrophylla is one of the most commonly used citrus rootstocks and it was found exhibiting witches’ broom disease symptoms. Polymerase chain reaction (PCR) was used to amplify the 16S ribosomal gene of phytoplasmas with the universal primers P1/P7 as a direct PCR and R16F2n / R16R2 primers for the nested PCR. The amplifications from all the symptomatic plants yielded a product of 1.8 kb and 1.2 kb in the direct and the nested PCR, respectively. Sequence identity results from BLAST and phylogenetic analysis of the 16S rRNA gene sequences confirmed that the phytoplasma identified in C. macrophylla in Oman is a ‘Candidatus Phytoplasma aurantifolia’-related strain.

Original language	English
Pages (from-to)	7-8
Number of pages	2
Journal	Phytopathogenic Mollicutes
Volume	9
Issue number	1
DOIs	https://doi.org/10.5958/2249-4677.2019.00004.5
Publication status	Published - Jun 2019
Externally published	Yes

Keywords

Citrus macrophylla
Oman
Phytoplasma
WBDL
Witches’ broom

ASJC Scopus subject areas

Infectious Diseases
Microbiology (medical)
Cell Biology
Ecology, Evolution, Behavior and Systematics
Parasitology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.5958/2249-4677.2019.00004.5

Cite this

@article{30b3084aaacb4101bf8edab207ec91d1,

title = "First report of a {\textquoteleft}candidatus phytoplasma aurantifolia{\textquoteright}-related strain in citrus macrophylla in Oman",

abstract = "Citrus macrophylla is one of the most commonly used citrus rootstocks and it was found exhibiting witches{\textquoteright} broom disease symptoms. Polymerase chain reaction (PCR) was used to amplify the 16S ribosomal gene of phytoplasmas with the universal primers P1/P7 as a direct PCR and R16F2n / R16R2 primers for the nested PCR. The amplifications from all the symptomatic plants yielded a product of 1.8 kb and 1.2 kb in the direct and the nested PCR, respectively. Sequence identity results from BLAST and phylogenetic analysis of the 16S rRNA gene sequences confirmed that the phytoplasma identified in C. macrophylla in Oman is a {\textquoteleft}Candidatus Phytoplasma aurantifolia{\textquoteright}-related strain.",

keywords = "Citrus macrophylla, Oman, Phytoplasma, WBDL, Witches{\textquoteright} broom",

author = "Al-Subhi, {Ali M.} and Al-Yahyai, {Rashid A.} and Al-Sadi, {Abdullah M.}",

year = "2019",

month = jun,

doi = "10.5958/2249-4677.2019.00004.5",

language = "English",

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pages = "7--8",

journal = "Phytopathogenic Mollicutes",

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publisher = "Technology Society of Basic and Applied Sciences",

number = "1",

}

TY - JOUR

T1 - First report of a ‘candidatus phytoplasma aurantifolia’-related strain in citrus macrophylla in Oman

AU - Al-Subhi, Ali M.

AU - Al-Yahyai, Rashid A.

AU - Al-Sadi, Abdullah M.

PY - 2019/6

Y1 - 2019/6

N2 - Citrus macrophylla is one of the most commonly used citrus rootstocks and it was found exhibiting witches’ broom disease symptoms. Polymerase chain reaction (PCR) was used to amplify the 16S ribosomal gene of phytoplasmas with the universal primers P1/P7 as a direct PCR and R16F2n / R16R2 primers for the nested PCR. The amplifications from all the symptomatic plants yielded a product of 1.8 kb and 1.2 kb in the direct and the nested PCR, respectively. Sequence identity results from BLAST and phylogenetic analysis of the 16S rRNA gene sequences confirmed that the phytoplasma identified in C. macrophylla in Oman is a ‘Candidatus Phytoplasma aurantifolia’-related strain.

AB - Citrus macrophylla is one of the most commonly used citrus rootstocks and it was found exhibiting witches’ broom disease symptoms. Polymerase chain reaction (PCR) was used to amplify the 16S ribosomal gene of phytoplasmas with the universal primers P1/P7 as a direct PCR and R16F2n / R16R2 primers for the nested PCR. The amplifications from all the symptomatic plants yielded a product of 1.8 kb and 1.2 kb in the direct and the nested PCR, respectively. Sequence identity results from BLAST and phylogenetic analysis of the 16S rRNA gene sequences confirmed that the phytoplasma identified in C. macrophylla in Oman is a ‘Candidatus Phytoplasma aurantifolia’-related strain.

KW - Citrus macrophylla

KW - Oman

KW - Phytoplasma

KW - WBDL

KW - Witches’ broom

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First report of a ‘candidatus phytoplasma aurantifolia’-related strain in citrus macrophylla in Oman

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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