TY - JOUR
T1 - First report of 16SrII-D phytoplasmas in Verbesina encelioides showing phyllody in Oman
AU - Al-Subhi, Ali M.
AU - Al-Alwai, Ala’A K.
AU - Al-Yahyai, Rashid A.
AU - Al-Sadi, Abdullah M.
N1 - Publisher Copyright:
© 2023, Technology Society of Basic and Applied Sciences. All rights reserved.
PY - 2023/1/1
Y1 - 2023/1/1
N2 - Verbesina encelioides, Asteraceae, in the sunflower family, is commonly known as golden crownbeard. It was found in Oman developing phyllody symptoms, a phytoplasma-associated disease. Polymerase chain reaction (PCR) was used to amplify the 16S rRNA, secA, imp and SAP11 genes of phytoplasmas using direct and nested techniques. The PCR amplifications from all infected plants was amplifying expected length fragments in both direct and the nested PCR from symptomatic plant samples. Sequence identity results on BLAST and phylogenetic analyses of these genes sequences confirm that the phytoplasma detected in V. encelioides is a member 16SrII-D subgroup and shares more than 99.5% of all four genes sequence identity to the other phytoplasmas enclosed in this ribosomal subgroup.
AB - Verbesina encelioides, Asteraceae, in the sunflower family, is commonly known as golden crownbeard. It was found in Oman developing phyllody symptoms, a phytoplasma-associated disease. Polymerase chain reaction (PCR) was used to amplify the 16S rRNA, secA, imp and SAP11 genes of phytoplasmas using direct and nested techniques. The PCR amplifications from all infected plants was amplifying expected length fragments in both direct and the nested PCR from symptomatic plant samples. Sequence identity results on BLAST and phylogenetic analyses of these genes sequences confirm that the phytoplasma detected in V. encelioides is a member 16SrII-D subgroup and shares more than 99.5% of all four genes sequence identity to the other phytoplasmas enclosed in this ribosomal subgroup.
KW - PCR
KW - RFLP
KW - molecular characterization
KW - plant disease
KW - sequencing
UR - http://www.scopus.com/inward/record.url?scp=85163588741&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85163588741&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/06001a8d-4a75-3ee7-bb9a-9a68172e1964/
U2 - 10.5958/2249-4677.2023.00061.0
DO - 10.5958/2249-4677.2023.00061.0
M3 - Article
AN - SCOPUS:85163588741
SN - 2249-4669
VL - 13
SP - 121
EP - 122
JO - Phytopathogenic Mollicutes
JF - Phytopathogenic Mollicutes
IS - 1
ER -