First record in Upper Egypt of vascular wilt on pomegranate caused by Fusarium oxysporum, its molecular identification and artificial pathogenicity

Ramadan A. Mohamed; Osama A. Al-Bedak; Sedky H.A. Hassan

doi:10.1007/s41348-020-00385-z

First record in Upper Egypt of vascular wilt on pomegranate caused by Fusarium oxysporum, its molecular identification and artificial pathogenicity

Ramadan A. Mohamed, Osama A. Al-Bedak, Sedky H.A. Hassan^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

A severe vascular pomegranate wilt (Punica granatum L.) has recently been discovered and reported for the first time in Upper Egypt. Diseased samples were obtained from an infected farm in El-Badari city, Assiut Governorate, Egypt, and the pathogen was isolated on potato dextrose agar at 25 °C. Phenotypic features and DNA sequencing of the pathogen's internal transcribed spacer region showed that the fungus was Fusarium oxysporum. The pathogenicity study conducted with 2-year-old plants resulted in 80% infection. F. oxysporum re-isolated from all the inoculated pomegranate trees exhibited the same morphological characters as those observed among the isolates.

Original language	English
Pages (from-to)	311-316
Number of pages	6
Journal	Journal of Plant Diseases and Protection
Volume	128
Issue number	1
DOIs	https://doi.org/10.1007/s41348-020-00385-z
Publication status	Published - Feb 2021
Externally published	Yes

Keywords

Fusarium oxysporum
Pathogenicity
Pomegranate
Vascular wilt

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science
Horticulture

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10.1007/s41348-020-00385-z

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title = "First record in Upper Egypt of vascular wilt on pomegranate caused by Fusarium oxysporum, its molecular identification and artificial pathogenicity",

abstract = "A severe vascular pomegranate wilt (Punica granatum L.) has recently been discovered and reported for the first time in Upper Egypt. Diseased samples were obtained from an infected farm in El-Badari city, Assiut Governorate, Egypt, and the pathogen was isolated on potato dextrose agar at 25 °C. Phenotypic features and DNA sequencing of the pathogen's internal transcribed spacer region showed that the fungus was Fusarium oxysporum. The pathogenicity study conducted with 2-year-old plants resulted in 80% infection. F. oxysporum re-isolated from all the inoculated pomegranate trees exhibited the same morphological characters as those observed among the isolates.",

keywords = "Fusarium oxysporum, Pathogenicity, Pomegranate, Vascular wilt",

author = "Mohamed, {Ramadan A.} and Al-Bedak, {Osama A.} and Hassan, {Sedky H.A.}",

note = "Funding Information: This work was financially supported by the Academy of Scientific Research & Technology (ASRT), Egypt, Grant No. 6371 under the project Science Up. The authors greatly acknowledge the kind help and the keen assistance of all staff of the Assiut University Mycological Centre (AUMC). Publisher Copyright: {\textcopyright} 2020, Deutsche Phytomedizinische Gesellschaft.",

year = "2021",

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doi = "10.1007/s41348-020-00385-z",

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AU - Mohamed, Ramadan A.

AU - Al-Bedak, Osama A.

AU - Hassan, Sedky H.A.

N1 - Funding Information: This work was financially supported by the Academy of Scientific Research & Technology (ASRT), Egypt, Grant No. 6371 under the project Science Up. The authors greatly acknowledge the kind help and the keen assistance of all staff of the Assiut University Mycological Centre (AUMC). Publisher Copyright: © 2020, Deutsche Phytomedizinische Gesellschaft.

PY - 2021/2

Y1 - 2021/2

N2 - A severe vascular pomegranate wilt (Punica granatum L.) has recently been discovered and reported for the first time in Upper Egypt. Diseased samples were obtained from an infected farm in El-Badari city, Assiut Governorate, Egypt, and the pathogen was isolated on potato dextrose agar at 25 °C. Phenotypic features and DNA sequencing of the pathogen's internal transcribed spacer region showed that the fungus was Fusarium oxysporum. The pathogenicity study conducted with 2-year-old plants resulted in 80% infection. F. oxysporum re-isolated from all the inoculated pomegranate trees exhibited the same morphological characters as those observed among the isolates.

AB - A severe vascular pomegranate wilt (Punica granatum L.) has recently been discovered and reported for the first time in Upper Egypt. Diseased samples were obtained from an infected farm in El-Badari city, Assiut Governorate, Egypt, and the pathogen was isolated on potato dextrose agar at 25 °C. Phenotypic features and DNA sequencing of the pathogen's internal transcribed spacer region showed that the fungus was Fusarium oxysporum. The pathogenicity study conducted with 2-year-old plants resulted in 80% infection. F. oxysporum re-isolated from all the inoculated pomegranate trees exhibited the same morphological characters as those observed among the isolates.

KW - Fusarium oxysporum

KW - Pathogenicity

KW - Pomegranate

KW - Vascular wilt

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First record in Upper Egypt of vascular wilt on pomegranate caused by Fusarium oxysporum, its molecular identification and artificial pathogenicity

Abstract

Keywords

ASJC Scopus subject areas

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