Expression profiling of DUF599 genes revealed their role in regulating abiotic stress response in solanum tuberosum

Madiha Zaynab, Hamed A. Ghramh, Yasir Sharif, Sajid Fiaz, Rashid Al-Yahyai, Maryam A. Alahdal, Sameer H. Qari, Kamel Hessini, Xiazi Huang*, Shuangfei Li

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


The proteins containing the domain of unknown functions (DUFs) have significant roles in stress response and the growth of plants. A comprehensive genome-wide analysis and expression profiling of DUF599 was conducted to identify their roles in potato growth and response to stressed conditions. A total of nine DUF599 genes were identified, located on five chromosomes in the potato genome. The phylogenetic analysis divided StDUF599 genes into three groups in accordance with gene conserved motifs and gene structure distribution patterns. The StDUF599 promoters comprised several cis-acting factors responsive to plant hormones and abiotic stresses. The present study revealed that StDUF599 genes also possessed MBS, LTR, ABRE, and anaerobic induction responsive elements, indicating their importance in coping with abiotic stresses. StDUF599 genes were the target of several families of micro-RNAs also identified in this study. Purifying selection pressures lead to the duplication of the StDUF599 genes. Expression analysis of StDUF599-6 and StDUF599-9 in various tissues illustrated their vital role in developmental processes. It was found that StDUF599-7 and StDUF599-9 were highly expressed against heat and salt stresses. Expression profiling revealed that the StDUF599-8 gene has a significant role against GA3 and IAA. Lastly, this article forecasted that the DUF599 genes could enhance plant tolerance against several abiotic stresses.

Original languageEnglish
Article number102368
JournalJournal of King Saud University - Science
Issue number1
Publication statusPublished - Jan 2023


  • Abiotic stress
  • Duplication
  • Gene expression
  • Phylogeny
  • Phytohormones
  • miRNAs

ASJC Scopus subject areas

  • General

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