Drilling in bone is a common surgical procedure routinely performed in orthopedics and dental surgeries for repair and fixation purposes. Measurement and control of bone temperature and drilling thrust force are critical to the outcome of the procedure. Excessive heat and large drilling force and torque produce in bone drilling process may cause physiological changes in the bone cells. The aim of this study was to evaluate the extent of biological damage in the immediate vicinity of the drilling region. Temperature in bone drilling process was measured by varying drill speed. The effect of bone temperature on the extent of cells damage surrounding the drilling area was evaluated. Necrotic depth was measured for the range of temperatures obtained from drilling experiments. Elevated temperature in bone was found to have negative impact on the health of the bone. Result showed that minimum cell damage can be achieved by using lower drill speed in bone drilling operation.