Eight new microsatellite markers in Atlantic cod (Gadus morhua L.) derived from an enriched genomic library

Mette Serine Wesmajervi; Tekle Tafese; Jørgen Stenvik; Kjersti Turid Fjalestad; Børge Damsgård; Madjid Delghandi

doi:10.1111/j.1471-8286.2006.01555.x

Eight new microsatellite markers in Atlantic cod (Gadus morhua L.) derived from an enriched genomic library

Mette Serine Wesmajervi, Tekle Tafese, Jørgen Stenvik, Kjersti Turid Fjalestad, Børge Damsgård, Madjid Delghandi^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

One hundred and fifty random clones from an enriched genomic library of Atlantic cod were sequenced. Primer pairs were designed for 15 microsatellites containing perfect di-, tri-, tetra- and hexanucleotide repeats and characterized in 96 unrelated fish. Eight markers were successfully amplified, with the number of alleles ranging from two to nine per locus and observed heterozygosity ranging from 0.341 to 0.977. Loci Gmo-G13 and Gmo-G14 had a significant excess of homozygotes. All loci conformed to the Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed no significant departure from the null hypothesis between any of the loci.

Original language	English
Pages (from-to)	138-140
Number of pages	3
Journal	Molecular Ecology Notes
Volume	7
Issue number	1
DOIs	https://doi.org/10.1111/j.1471-8286.2006.01555.x
Publication status	Published - Jan 2007
Externally published	Yes

Keywords

Atlantic cod
Enriched genomic library
Microsatellites
Type II markers

ASJC Scopus subject areas

Biochemistry
Ecology
Biochemistry, Genetics and Molecular Biology(all)

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10.1111/j.1471-8286.2006.01555.x

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abstract = "One hundred and fifty random clones from an enriched genomic library of Atlantic cod were sequenced. Primer pairs were designed for 15 microsatellites containing perfect di-, tri-, tetra- and hexanucleotide repeats and characterized in 96 unrelated fish. Eight markers were successfully amplified, with the number of alleles ranging from two to nine per locus and observed heterozygosity ranging from 0.341 to 0.977. Loci Gmo-G13 and Gmo-G14 had a significant excess of homozygotes. All loci conformed to the Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed no significant departure from the null hypothesis between any of the loci.",

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AU - Wesmajervi, Mette Serine

AU - Tafese, Tekle

AU - Stenvik, Jørgen

AU - Fjalestad, Kjersti Turid

AU - Damsgård, Børge

AU - Delghandi, Madjid

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KW - Type II markers

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Eight new microsatellite markers in Atlantic cod (Gadus morhua L.) derived from an enriched genomic library

Abstract

Keywords

ASJC Scopus subject areas

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