Diagnostic Mr 31/32000 proteins of Schistosoma mansoni (Sm31/32) and S. haematobium (Sh31/32): Stability and reaction conditions for prospective field tests

Mohamed A. Idris

doi:10.1017/S0022149X0001052X

Diagnostic Mr 31/32000 proteins of Schistosoma mansoni (Sm31/32) and S. haematobium (Sh31/32): Stability and reaction conditions for prospective field tests

Mohamed A. Idris

Microbiology & Immunology

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The existence of adult Schistosoma haematobium worm proteins (Sh31/32) homologous to the diagnostic Sm3l/32 of S. mansoni is shown. Sm31/32 and Sh31/32, adsorbed on nitrocellulose and kept dry on the bench before immunoblot analysis, were antigenically stable for at least 4 years including storage for 17 months in tropical climates. The antigens react with patient sera in the absence of defined buffers under rather simple conditions (“humid chamber blot”). The results add to the use of these antigens for serodiagnosis of schistosomiasis in endemic areas.

Original language	English
Pages (from-to)	89-94
Number of pages	6
Journal	Journal of Helminthology
Volume	65
Issue number	2
DOIs	https://doi.org/10.1017/S0022149X0001052X
Publication status	Published - Jun 1991

Keywords

Schistosoma mansoni
Sm31/32
diagnostic proteins
reaction conditions Schistosoma haematobium Sh 31/32
stability

ASJC Scopus subject areas

Parasitology
Animal Science and Zoology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1017/S0022149X0001052X

Cite this

@article{80ec583b2860405e935e17e1e4922557,

title = "Diagnostic Mr 31/32000 proteins of Schistosoma mansoni (Sm31/32) and S. haematobium (Sh31/32): Stability and reaction conditions for prospective field tests",

abstract = "The existence of adult Schistosoma haematobium worm proteins (Sh31/32) homologous to the diagnostic Sm3l/32 of S. mansoni is shown. Sm31/32 and Sh31/32, adsorbed on nitrocellulose and kept dry on the bench before immunoblot analysis, were antigenically stable for at least 4 years including storage for 17 months in tropical climates. The antigens react with patient sera in the absence of defined buffers under rather simple conditions (“humid chamber blot”). The results add to the use of these antigens for serodiagnosis of schistosomiasis in endemic areas.",

keywords = "Schistosoma mansoni, Sm31/32, diagnostic proteins, reaction conditions Schistosoma haematobium Sh 31/32, stability",

author = "Idris, {Mohamed A.}",

note = "Funding Information: We would like to thank Dr Suad M. Sulaiman (Bilharzia Research Unit, National Health Laboratories, Khartoum, Sudan) for invaluable help with infection of monkeys and perfusion of 5. haematobium and Mrs Petra Numrich for excellent technical assistance. This investigation received financial assistance from the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (ID-No. 880226), the Deutscher Akademischer Austauschdienst, the Krebs-und Scharlachstiftung and the Morsel-Stiftung.",

year = "1991",

month = jun,

doi = "10.1017/S0022149X0001052X",

language = "English",

volume = "65",

pages = "89--94",

journal = "Journal of Helminthology",

issn = "0022-149X",

publisher = "Cambridge University Press",

number = "2",

}

TY - JOUR

T1 - Diagnostic Mr 31/32000 proteins of Schistosoma mansoni (Sm31/32) and S. haematobium (Sh31/32)

T2 - Stability and reaction conditions for prospective field tests

AU - Idris, Mohamed A.

N1 - Funding Information: We would like to thank Dr Suad M. Sulaiman (Bilharzia Research Unit, National Health Laboratories, Khartoum, Sudan) for invaluable help with infection of monkeys and perfusion of 5. haematobium and Mrs Petra Numrich for excellent technical assistance. This investigation received financial assistance from the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (ID-No. 880226), the Deutscher Akademischer Austauschdienst, the Krebs-und Scharlachstiftung and the Morsel-Stiftung.

PY - 1991/6

Y1 - 1991/6

N2 - The existence of adult Schistosoma haematobium worm proteins (Sh31/32) homologous to the diagnostic Sm3l/32 of S. mansoni is shown. Sm31/32 and Sh31/32, adsorbed on nitrocellulose and kept dry on the bench before immunoblot analysis, were antigenically stable for at least 4 years including storage for 17 months in tropical climates. The antigens react with patient sera in the absence of defined buffers under rather simple conditions (“humid chamber blot”). The results add to the use of these antigens for serodiagnosis of schistosomiasis in endemic areas.

AB - The existence of adult Schistosoma haematobium worm proteins (Sh31/32) homologous to the diagnostic Sm3l/32 of S. mansoni is shown. Sm31/32 and Sh31/32, adsorbed on nitrocellulose and kept dry on the bench before immunoblot analysis, were antigenically stable for at least 4 years including storage for 17 months in tropical climates. The antigens react with patient sera in the absence of defined buffers under rather simple conditions (“humid chamber blot”). The results add to the use of these antigens for serodiagnosis of schistosomiasis in endemic areas.

KW - Schistosoma mansoni

KW - Sm31/32

KW - diagnostic proteins

KW - reaction conditions Schistosoma haematobium Sh 31/32

KW - stability

UR - http://www.scopus.com/inward/record.url?scp=0025914176&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025914176&partnerID=8YFLogxK

U2 - 10.1017/S0022149X0001052X

DO - 10.1017/S0022149X0001052X

M3 - Article

C2 - 1908874

AN - SCOPUS:0025914176

SN - 0022-149X

VL - 65

SP - 89

EP - 94

JO - Journal of Helminthology

JF - Journal of Helminthology

IS - 2

ER -

Diagnostic Mr 31/32000 proteins of Schistosoma mansoni (Sm31/32) and S. haematobium (Sh31/32): Stability and reaction conditions for prospective field tests

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this