TY - JOUR
T1 - Development of a Tacrolimus-loaded carboxymethyl chitosan scaffold as an effective 3D-printed wound dressing
AU - Al-Hashmi, Sulaiman
AU - Vakilian, Saeid
AU - Jamshidi-adegani, Fatemeh
AU - Al-Kindi, Juhaina
AU - Al-Fahdi, Fahad
AU - Al-Hatmi, Abdullah M.S.
AU - Al-Jahdhami, Habib
AU - Anwar, Muhammad U.
AU - Al-Wahaibi, Nasar
AU - Shalaby, Asem
AU - Al-Harrasi, Ahmed
N1 - Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2023/9/1
Y1 - 2023/9/1
N2 - Infection and lack of angiogenesis are considered to be the main factors slowing the healing process in chronic wounds. To address these challenges, bioactive, multi-functional wound dressings have received a lot of attention. A 3D-printed carboxymethyl chitosan scaffold loaded with Tacrolimus (TAC-CMC) was introduced and tested as a potential bioactive dressing for chronic wound healing. Following topographical and physical characterization of the wound dressings, the release profile of Tacrolimus from the TAC-CMC scaffold was investigated. In vitro evaluation revealed that both carboxymethyl chitosan scaffold (CMC) and TAC-CMC scaffolds were biocompatible, but only TAC-CMC causing an increase in the secretion of vascular endothelial growth factor (VEGF) from fibroblasts. Disk diffusion test of the fabricated scaffolds demonstrated a significant antibacterial activity of TAC-CMC scaffold against both E. Coli and S. aureus as Gram negative and positive bacteria, respectively. The in vivo assessments of the bare and bioactive wound dressings have revealed that 7 days post-wounding, wounds treated with the TAC-CMC resulted in a 90.4 ± 2.4% closure rate which was significantly faster than those in the positive control (Comfeel plus®). The histopathological evaluation of the treated and non-treated wounds proved the efficacy of TAC-CMC scaffold in improving angiogenesis, epidermal regeneration, fibroblasts proliferation, and inflammatory responses.
AB - Infection and lack of angiogenesis are considered to be the main factors slowing the healing process in chronic wounds. To address these challenges, bioactive, multi-functional wound dressings have received a lot of attention. A 3D-printed carboxymethyl chitosan scaffold loaded with Tacrolimus (TAC-CMC) was introduced and tested as a potential bioactive dressing for chronic wound healing. Following topographical and physical characterization of the wound dressings, the release profile of Tacrolimus from the TAC-CMC scaffold was investigated. In vitro evaluation revealed that both carboxymethyl chitosan scaffold (CMC) and TAC-CMC scaffolds were biocompatible, but only TAC-CMC causing an increase in the secretion of vascular endothelial growth factor (VEGF) from fibroblasts. Disk diffusion test of the fabricated scaffolds demonstrated a significant antibacterial activity of TAC-CMC scaffold against both E. Coli and S. aureus as Gram negative and positive bacteria, respectively. The in vivo assessments of the bare and bioactive wound dressings have revealed that 7 days post-wounding, wounds treated with the TAC-CMC resulted in a 90.4 ± 2.4% closure rate which was significantly faster than those in the positive control (Comfeel plus®). The histopathological evaluation of the treated and non-treated wounds proved the efficacy of TAC-CMC scaffold in improving angiogenesis, epidermal regeneration, fibroblasts proliferation, and inflammatory responses.
KW - 3D-printing
KW - Angiogenesis
KW - Antibacterial
KW - Carboxymethyl chitosan
KW - Tacrolimus
KW - Wound dressing
UR - http://www.scopus.com/inward/record.url?scp=85164336937&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85164336937&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/9aff2898-6612-3759-9983-27c74751b00c/
U2 - 10.1016/j.jddst.2023.104707
DO - 10.1016/j.jddst.2023.104707
M3 - Article
AN - SCOPUS:85164336937
SN - 1773-2247
VL - 86
JO - Journal of Drug Delivery Science and Technology
JF - Journal of Drug Delivery Science and Technology
M1 - 104707
ER -