Developing efficient thermophilic cellulose degrading consortium for glucose production from different agro-residues

Saurabh Singh, Durgesh Kumar Jaiswal, Nallusamy Sivakumar, Jay Prakash Verma*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


In the present study, 11 cellulose degrading bacterial strains were isolated from water and soil samples of hot springs in the Chumathang village, Leh and Ladakh region, India. The isolated strains were identified as Bacillus subtilis, Bacillus aryabhattai, Bacillus stratosphericus, Bacillus altitudinis, and Brevibacterium frigoritolerans by biochemical and molecular approaches. All the strains were evaluated for the total cellulase, endoglucanase, exoglucanase, and β-glucosidase enzyme activities. On the basis of overall individual cellulose degrading enzyme activities, three strains were selected to develop consortium to enhance their cellulase enzyme activities. The potent cellulose degraders B. stratosphericus BHUJPV-H5, B. subtilis BHUJPV-H19, and B. subtilis BHUJPV-H12 were selected for the consortium development. The effect of cellulase activities of bacterial strains were evaluated ranged up to 6.06 and 0.72mg ml-1 glucose by agro-residues of sugarcane bagasse and wheat straw, respectively, after 1 h of incubation. Total cellulase enzyme activity of consortia was recorded two times higher than the individual organisms. These strains can be used for enhancing bioethanol production from lignocellulosic biomass, which can consequently boost biofuel production industry.

Original languageEnglish
Article number61
JournalFrontiers in Energy Research
Issue numberJUL
Publication statusPublished - 2019


  • B-glucosidase
  • Cellulase
  • Consortium
  • Endoglucanase
  • Exoglucanase
  • Glucose
  • Sugarcane bagasse
  • Wheat straw

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Energy Engineering and Power Technology
  • Economics and Econometrics


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