Detoxification of aflatoxin B1 by an aqueous extract from leaves of Adhatoda vasica Nees

S. Vijayanandraj; R. Brinda; K. Kannan; R. Adhithya; S. Vinothini; K. Senthil; Ramakoteswara Rao Chinta; V. Paranidharan; R. Velazhahan

doi:10.1016/j.micres.2013.07.008

Detoxification of aflatoxin B1 by an aqueous extract from leaves of Adhatoda vasica Nees

S. Vijayanandraj, R. Brinda, K. Kannan, R. Adhithya, S. Vinothini, K. Senthil, Ramakoteswara Rao Chinta, V. Paranidharan, R. Velazhahan^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

75 Citations (Scopus)

Abstract

The effectiveness of aqueous extracts of various medicinal plants in detoxification of aflatoxin B1 (AFB1) was tested in vitro by thin-layer chromatography and enzyme-linked immunosorbent assay (ELISA). Among the different plant extracts, the leaf extract of Vasaka (Adhatoda vasica Nees) showed the maximum degradation of AFB1 (≥98%) after incubation for 24. h at 37. °C. The aflatoxin detoxifying activity of the A. vasica leaf extract was significantly reduced by heating to 100. °C for 10. min or autoclaving at 121. °C for 20. min. Dialysis had no effect on aflatoxin detoxifying ability of A. vasica extract and the dialyzed extract showed similar level of detoxification of AFB1 as that of the untreated extract. A time course study of aflatoxin detoxification by A. vasica extract showed that 69% of the toxin was degraded within 6. h and ≥95% degradation was observed after 24. h of incubation. Detoxification of AFB1 by A. vasica extract was further confirmed by liquid chromatography-mass spectrometry (LC-MS) analysis. Phytochemical analysis revealed the presence of alkaloids in methanolic extract of A. vasica leaves. A partially purified alkaloid from A. vasica leaves by preparative TLC exhibited strong AFB1 detoxification activity.

Original language	English
Pages (from-to)	294-300
Number of pages	7
Journal	Microbiological Research
Volume	169
Issue number	4
DOIs	https://doi.org/10.1016/j.micres.2013.07.008
Publication status	Published - Apr 2014
Externally published	Yes

Keywords

Adhatoda vasica
Aflatoxin B1
Detoxification
Medicinal plant

ASJC Scopus subject areas

Microbiology

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10.1016/j.micres.2013.07.008

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@article{a5b7e144870b4f9bb1ac11ad468d6c59,

title = "Detoxification of aflatoxin B1 by an aqueous extract from leaves of Adhatoda vasica Nees",

abstract = "The effectiveness of aqueous extracts of various medicinal plants in detoxification of aflatoxin B1 (AFB1) was tested in vitro by thin-layer chromatography and enzyme-linked immunosorbent assay (ELISA). Among the different plant extracts, the leaf extract of Vasaka (Adhatoda vasica Nees) showed the maximum degradation of AFB1 (≥98%) after incubation for 24. h at 37. °C. The aflatoxin detoxifying activity of the A. vasica leaf extract was significantly reduced by heating to 100. °C for 10. min or autoclaving at 121. °C for 20. min. Dialysis had no effect on aflatoxin detoxifying ability of A. vasica extract and the dialyzed extract showed similar level of detoxification of AFB1 as that of the untreated extract. A time course study of aflatoxin detoxification by A. vasica extract showed that 69% of the toxin was degraded within 6. h and ≥95% degradation was observed after 24. h of incubation. Detoxification of AFB1 by A. vasica extract was further confirmed by liquid chromatography-mass spectrometry (LC-MS) analysis. Phytochemical analysis revealed the presence of alkaloids in methanolic extract of A. vasica leaves. A partially purified alkaloid from A. vasica leaves by preparative TLC exhibited strong AFB1 detoxification activity.",

keywords = "Adhatoda vasica, Aflatoxin B1, Detoxification, Medicinal plant",

author = "S. Vijayanandraj and R. Brinda and K. Kannan and R. Adhithya and S. Vinothini and K. Senthil and Chinta, {Ramakoteswara Rao} and V. Paranidharan and R. Velazhahan",

note = "Funding Information: We thank Prof. S. Muthukrishnan, Department of Biochemistry, Kansas State University, Manhattan, Kansas, USA for critical review of this manuscript. This work was supported in part by the Department of Science and Technology, Government of India .",

year = "2014",

month = apr,

doi = "10.1016/j.micres.2013.07.008",

language = "English",

volume = "169",

pages = "294--300",

journal = "Microbiological Research",

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TY - JOUR

T1 - Detoxification of aflatoxin B1 by an aqueous extract from leaves of Adhatoda vasica Nees

AU - Vijayanandraj, S.

AU - Brinda, R.

AU - Kannan, K.

AU - Adhithya, R.

AU - Vinothini, S.

AU - Senthil, K.

AU - Chinta, Ramakoteswara Rao

AU - Paranidharan, V.

AU - Velazhahan, R.

N1 - Funding Information: We thank Prof. S. Muthukrishnan, Department of Biochemistry, Kansas State University, Manhattan, Kansas, USA for critical review of this manuscript. This work was supported in part by the Department of Science and Technology, Government of India .

PY - 2014/4

Y1 - 2014/4

N2 - The effectiveness of aqueous extracts of various medicinal plants in detoxification of aflatoxin B1 (AFB1) was tested in vitro by thin-layer chromatography and enzyme-linked immunosorbent assay (ELISA). Among the different plant extracts, the leaf extract of Vasaka (Adhatoda vasica Nees) showed the maximum degradation of AFB1 (≥98%) after incubation for 24. h at 37. °C. The aflatoxin detoxifying activity of the A. vasica leaf extract was significantly reduced by heating to 100. °C for 10. min or autoclaving at 121. °C for 20. min. Dialysis had no effect on aflatoxin detoxifying ability of A. vasica extract and the dialyzed extract showed similar level of detoxification of AFB1 as that of the untreated extract. A time course study of aflatoxin detoxification by A. vasica extract showed that 69% of the toxin was degraded within 6. h and ≥95% degradation was observed after 24. h of incubation. Detoxification of AFB1 by A. vasica extract was further confirmed by liquid chromatography-mass spectrometry (LC-MS) analysis. Phytochemical analysis revealed the presence of alkaloids in methanolic extract of A. vasica leaves. A partially purified alkaloid from A. vasica leaves by preparative TLC exhibited strong AFB1 detoxification activity.

AB - The effectiveness of aqueous extracts of various medicinal plants in detoxification of aflatoxin B1 (AFB1) was tested in vitro by thin-layer chromatography and enzyme-linked immunosorbent assay (ELISA). Among the different plant extracts, the leaf extract of Vasaka (Adhatoda vasica Nees) showed the maximum degradation of AFB1 (≥98%) after incubation for 24. h at 37. °C. The aflatoxin detoxifying activity of the A. vasica leaf extract was significantly reduced by heating to 100. °C for 10. min or autoclaving at 121. °C for 20. min. Dialysis had no effect on aflatoxin detoxifying ability of A. vasica extract and the dialyzed extract showed similar level of detoxification of AFB1 as that of the untreated extract. A time course study of aflatoxin detoxification by A. vasica extract showed that 69% of the toxin was degraded within 6. h and ≥95% degradation was observed after 24. h of incubation. Detoxification of AFB1 by A. vasica extract was further confirmed by liquid chromatography-mass spectrometry (LC-MS) analysis. Phytochemical analysis revealed the presence of alkaloids in methanolic extract of A. vasica leaves. A partially purified alkaloid from A. vasica leaves by preparative TLC exhibited strong AFB1 detoxification activity.

KW - Adhatoda vasica

KW - Aflatoxin B1

KW - Detoxification

KW - Medicinal plant

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SN - 0944-5013

VL - 169

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EP - 300

JO - Microbiological Research

JF - Microbiological Research

IS - 4

ER -

Detoxification of aflatoxin B1 by an aqueous extract from leaves of Adhatoda vasica Nees

Abstract

Keywords

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