Detection of low plasma estradiol concentrations in nesting green turtles (Chelonia mydas) by HPLC/Ms-Ms

I. Y. Mahmoud, A. Y. Alkindi, T. Khan, S. N. Al-Bahry*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


In previous studies on nesting green turtles under natural conditions from different geographical regions, 17-β-estradiol (E2) was either undetectable or detected at very low levels. RIA and other related techniques were not sensitive enough to measure low E2 values in the green turtles. In this study, a sensitive method was used in detecting low hormone concentrations: high performance liquid chromatography with tandem quadruple mass spectrometry (HPLC-MS/MS). Using this technique, estradiol for the first time was detected in nesting green turtles during the peak season (June-October) at Ras Al-Hadd Reserve, Oman. The E2 values recorded from this study were the highest ever recorded from nesting green turtles in any geographical region, but the levels did not vary significantly throughout different phases of nesting. The presence of E2 during nesting presumably plays a role in the physiology and behavior of this species. Ras Al-Hadd hosts one of the largest nesting populations of green turtles in the world, and an understanding of their nesting patterns may be of value in conservation and management programs for this endangered species.

Original languageEnglish
Pages (from-to)170-174
Number of pages5
JournalJournal of Experimental Zoology Part A: Ecological Genetics and Physiology
Volume315 A
Issue number3
Publication statusPublished - Mar 1 2011

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Animal Science and Zoology
  • Molecular Biology
  • Genetics


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