TY - JOUR
T1 - Composition analysis and antimicrobial activity of essential oil from leaves of Laurus nobilis grown in Oman
AU - Al-Abri, Siham Saleh
AU - Said, Sadri Abdullah
AU - Touby, Salem Said Al
AU - Hossain, Mohammed Amzad
AU - Al-Sabahi, Jamal Nasser
N1 - Funding Information:
The authors are grateful to University of Nizwa for providing all chemicals and lab facilities. The authors are also grateful to SQU, Oman for the analysis of essential oil. Thanks to lab technicians who actively support to finish the work.
Publisher Copyright:
© 2022 The Author(s)
PY - 2022/11
Y1 - 2022/11
N2 - Traditionally, Laurus nobilis is used for the treatment of earaches and skin rashes as well as rheumatism. The most common technique to isolate the oil is hydro-distillation and steam-distillation. The investigation presented in this paper was aimed to unravel the antimicrobial activity and chemical composition of essential oil in the leaves of L. nobilis grown in Oman. The essential oil was extracted from the leave of the selected plant species by hydro distillation using Clevenger type apparatus. The antimicrobial assay was done by disc diffusion methods against three bacterial strains, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli, and one fungal strain: Candida albicans. The isolated essential oil was analyzed by gas chromatography-mass spectrometry (GC-MS). The percentage yield of the essential oil was 0.064% (w/w). The essential oil was found actively against the applied bacterial and fungal strains. The highest activity was found against S. aureus and the lowest was against E. coli, and the order was S. aureus > P. aeruginosa > E. coli. The essential oil was also active against the fungus C. albicans. The range of inhibition zones was 6–14 mm. The results of GC-MS analysis showed (E)-β-caryophyllene to be the major component about 59.62%. Other major components included α-selinene (14.03%), α-humulene (8.65%), β-selinene (4.99%), and α-pinene (4.98%). In addition, several minor components were found in the essential oil of L. nobilis. Based on the experimental results, it showed that the plant species that grown in Oman were a potential source of therapeutic agents. In conclusion, the essential oil and the plant could be used as natural and potential therapeutic agents to treat earaches, skin rashes, and rheumatism.
AB - Traditionally, Laurus nobilis is used for the treatment of earaches and skin rashes as well as rheumatism. The most common technique to isolate the oil is hydro-distillation and steam-distillation. The investigation presented in this paper was aimed to unravel the antimicrobial activity and chemical composition of essential oil in the leaves of L. nobilis grown in Oman. The essential oil was extracted from the leave of the selected plant species by hydro distillation using Clevenger type apparatus. The antimicrobial assay was done by disc diffusion methods against three bacterial strains, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli, and one fungal strain: Candida albicans. The isolated essential oil was analyzed by gas chromatography-mass spectrometry (GC-MS). The percentage yield of the essential oil was 0.064% (w/w). The essential oil was found actively against the applied bacterial and fungal strains. The highest activity was found against S. aureus and the lowest was against E. coli, and the order was S. aureus > P. aeruginosa > E. coli. The essential oil was also active against the fungus C. albicans. The range of inhibition zones was 6–14 mm. The results of GC-MS analysis showed (E)-β-caryophyllene to be the major component about 59.62%. Other major components included α-selinene (14.03%), α-humulene (8.65%), β-selinene (4.99%), and α-pinene (4.98%). In addition, several minor components were found in the essential oil of L. nobilis. Based on the experimental results, it showed that the plant species that grown in Oman were a potential source of therapeutic agents. In conclusion, the essential oil and the plant could be used as natural and potential therapeutic agents to treat earaches, skin rashes, and rheumatism.
KW - Antimicrobial activity
KW - Clevenger apparatus
KW - Essential oil
KW - Gas chromatography-mass spectrometry (GC-MS)
KW - Laurus nobilis
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U2 - 10.1016/j.jobab.2022.09.003
DO - 10.1016/j.jobab.2022.09.003
M3 - Article
SN - 2369-9698
VL - 7
SP - 328
EP - 334
JO - Journal of Bioresources and Bioproducts
JF - Journal of Bioresources and Bioproducts
IS - 4
ER -