TY - JOUR
T1 - Comparative study of the cytotoxicity, apoptotic, and epigenetic effects of Boswellic acid derivatives on breast cancer
AU - Jamshidi-adegani, Fatemeh
AU - Ghaemi, Shokoofeh
AU - Al-Hashmi, Sulaiman
AU - Vakilian, Saeid
AU - Al-kindi, Juhaina
AU - Rehman, Najeeb Ur
AU - Alam, Khurshid
AU - Al-Riyami, Khamis
AU - Csuk, Rene
AU - Arefian, Ehsan
AU - Al-Harrasi, Ahmed
N1 - Funding Information:
The authors gratefully acknowledge Mr. Saleh Al-Amri for the collection of oleo-gum resins of Boswellia sacra (B. sacra) from the Dhofar region (Oman). This research was funded by The Research Council external grant number BFP/RGP/HSS/18/024, 2019, and The GCC co-funding, grant number CL/SQU-GCC/17/03.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/11/21
Y1 - 2022/11/21
N2 - This study aimed to compare the effect of Boswellic acid derivatives on the viability, apoptosis, and epigenomic profiling of breast cancer. According to the viability assays, 3-O-acetyl-11-keto-β-Boswellic acid (AKBA) showed more toxicity against MDA-MB-231 cells when compared with the 3-O-acetyl-β-Boswellic acid (ABA). In contrast, ABA revealed less toxicity against MCF-10A. Cell cycle and apoptosis assays determined the maximum apoptotic effect of AKBA on MCF-7, and MDA-MB-231 cells. Interestingly, β-Boswellic acid (BA) and ABA did not promote the apoptosis in MCF-10A cells. Transwell migration assay indicated the greatest normalized inhibition (around 160%) in the migration of MDA-MB-231 cells induced by AKBA. The expression of P53, BAX, and BCL2 genes in cancerous cell lines has affirmed that both AKBA and ABA could induce the maximal apoptosis. Western-blot investigation demonstrated that the maximum over-expression of P53 protein (1.96 times) was caused by AKBA in MDA-MB-231 cells, followed by ABA in MCF-7 cells. The BCL2 protein expression was in agreement with the previously reported results. The global DNA methylation in both cancerous cells was reduced by ABA. These results suggest that ABA represented more epigenetic modulatory effect while AKBA shows more cytotoxic and apoptotic effect against breast cancer cell lines.
AB - This study aimed to compare the effect of Boswellic acid derivatives on the viability, apoptosis, and epigenomic profiling of breast cancer. According to the viability assays, 3-O-acetyl-11-keto-β-Boswellic acid (AKBA) showed more toxicity against MDA-MB-231 cells when compared with the 3-O-acetyl-β-Boswellic acid (ABA). In contrast, ABA revealed less toxicity against MCF-10A. Cell cycle and apoptosis assays determined the maximum apoptotic effect of AKBA on MCF-7, and MDA-MB-231 cells. Interestingly, β-Boswellic acid (BA) and ABA did not promote the apoptosis in MCF-10A cells. Transwell migration assay indicated the greatest normalized inhibition (around 160%) in the migration of MDA-MB-231 cells induced by AKBA. The expression of P53, BAX, and BCL2 genes in cancerous cell lines has affirmed that both AKBA and ABA could induce the maximal apoptosis. Western-blot investigation demonstrated that the maximum over-expression of P53 protein (1.96 times) was caused by AKBA in MDA-MB-231 cells, followed by ABA in MCF-7 cells. The BCL2 protein expression was in agreement with the previously reported results. The global DNA methylation in both cancerous cells was reduced by ABA. These results suggest that ABA represented more epigenetic modulatory effect while AKBA shows more cytotoxic and apoptotic effect against breast cancer cell lines.
KW - Epigenomics
KW - Tumor Suppressor Protein p53
KW - Proto-Oncogene Proteins c-bcl-2
KW - Epigenesis, Genetic
KW - Neoplasms
UR - http://www.scopus.com/inward/record.url?scp=85142352117&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85142352117&partnerID=8YFLogxK
U2 - 10.1038/s41598-022-24229-y
DO - 10.1038/s41598-022-24229-y
M3 - Article
C2 - 36411309
AN - SCOPUS:85142352117
SN - 2045-2322
VL - 12
SP - 19979
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 19979
ER -
