Circulating matrix metalloproteinase-9 and tissue inhibitors of metalloproteinases-1 and -2 levels in gestational hypertension

Muzahir H. Tayebjee, Ioannis Karalis, Sunil K. Nadar, D. Gareth Beevers, Robert J. MacFadyen, Gregory Y.H. Lip*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)


Background: Gestational hypertension (GH) is dangerous to both mother and child. Arterial invasiveness and growth are dependent on successful extracellular matrix (ECM) breakdown, which may be abnormal in GH. We hypothesized abnormalities in circulating matrix metalloproteinase-9 (MMP-9) and tissue inhibitors of metalloproteinases-1 and -2 (TIMP-1 and TIMP-2, respectively) in patients with GH, when compared with normotensive women with normal pregnancies and healthy nonpregnant control subjects. Methods: Plasma MMP-9, TIMP-1, and TIMP-2 were measured by ELISA in 23 women with GH, 30 normotensive pregnant women, and 28 nonpregnant women who were matched for age, gestational age, and parity. Results: Levels of circulating MMP-9, TIMP-1 and TIMP-2, and the MMP-9/TIMP-1 and MMP-9/TIMP-2 ratios were significantly different among the three groups (P =. 026, P =. 006, P =. 007, P =. 001 and P =. 008 respectively). Within the GH group, MMP-9 and the MMP-9/TIMP-1 ratio correlated negatively with age (r = -0.581, P =. 004 and r = -0.563, P =. 005, respectively) and levels of diastolic blood pressure (r = -0.432, P =. 040 and r = -0.461, P =. 027, respectively). With multiple regression analysis, only age independently correlated with circulating levels of MMP-9 (P =. 010); neither age nor levels of diastolic blood pressure had any effect on the MMP-9/TIMP-1 ratio. Conclusions: We have demonstrated altered MMP/TIMP ratios in maternal blood during GH. These observations suggest pregnancy-related changes in ECM breakdown and turnover. Given the importance of changes in ECM composition to vascular and cardiac structure in hypertension, we suggest that these observations may be related to the pathophysiology of human GH.

Original languageEnglish
Pages (from-to)325-329
Number of pages5
JournalAmerican Journal of Hypertension
Issue number3
Publication statusPublished - Mar 2005
Externally publishedYes


  • Gestational hypertension
  • Matrix metalloproteinases
  • Tissue inhibitor of metalloproteinases

ASJC Scopus subject areas

  • Internal Medicine


Dive into the research topics of 'Circulating matrix metalloproteinase-9 and tissue inhibitors of metalloproteinases-1 and -2 levels in gestational hypertension'. Together they form a unique fingerprint.

Cite this