Characterization of Carrageenan Extracted from Hypnea bryoides in Oman

Ahmed Ali Al-Alawi, Insaaf Mohammed Al-Marhubi, Mohammed Said Moosa Al-Belushi, Bassam Soussi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)


Carrageenophyte red seaweed from Oman, Hypnea bryoides, extracted using three different processes: an aqueous, a mild alkaline, and a more vigorous alkaline extraction was investigated. The resulting extract precipitated by alcohol was subject to chemical and rheological measurements. The total carbohydrate [ranged from 36.78 to 41.65 g/100 g], and ash [39.04 to 43.11 g/100 g] were the most abundant components in H. bryoides and contrary to the two, lipid content was found at a minimum [ranging from 2.95 to 3.38 g/100 g]. Alkali treatment with NaOH allowed complete conversion of kappa (κ) carrageenan form as detected by FTIR analysis. Total yield by alkali treatments gave higher yields (33%) compared with aqueous treatments (12%). However, subsequent aqueous treatment produced mixed carrageenan (μ and κ) with higher molecular weight compared with the alkali treatments which produced single carrageenan form (κ) with molecular weight of 4.1 × 10 5 Da. The effects of thermal history on gel-sol and sol-gel transition were investigated by differential scanning calorimeter (DSC) and rheology on a pure sample and 1.5% κ-carrageenan mixture added with 30 mM KCl. Transition temperatures from DSC and rheology showed comparable results and were in good agreement with those previously reported.

Original languageEnglish
Pages (from-to)893-899
Number of pages7
JournalMarine Biotechnology
Issue number5
Publication statusPublished - Oct 2011


  • Algae
  • FTIR
  • Hypnea bryoides
  • Phycocolloids
  • Seaweed

ASJC Scopus subject areas

  • Biotechnology
  • Aquatic Science


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