TY - JOUR
T1 - Avastin-Loaded 3D-Printed Alginate Scaffold as an Effective Antiadhesive Barrier to Prevent Postsurgical Adhesion Bands Formation
AU - Vakilian, Saeid
AU - Al-Hashmi, Sulaiman
AU - Al-kindi, Juhaina
AU - Al-Fahdi, Fahad
AU - Al-Wahaibi, Nasar
AU - Shalaby, Asem
AU - Al-Riyami, Hamad
AU - Al-Harrasi, Ahmed
AU - Jamshidi-adegani, Fatemeh
N1 - Publisher Copyright:
© 2024 Wiley-VCH GmbH.
PY - 2024/2/13
Y1 - 2024/2/13
N2 - Postoperative adhesion can cause complications, such as pain and organ blockage, in the abdominal regions. To address this issue, surgical techniques and antiadhesive treatments are applied. Given the significant role of vascularization in adhesion band formation, Avastin (Ava) that targets vascular endothelial growth factor (VEGF) can be applied to prevent peritoneal adhesion bands. Moreover, Alginate (Alg), a natural polysaccharide, is a promising physical barrier to prevent adhesion bands. Incorporating Ava into Alg hydrogel in a form of 3D-printed scaffold (Alg/Ava) has potential to suppress inflammation and angiogenesis, leading to reduce peritoneal adhesion bands. Following physical, morphological, and biocompatibility evaluations, the efficacy of Alg and Ava alone and their combination in Alg/Ava on the formation of postsurgical adhesions is evaluated. Upon confirming physical stability and sustained release of Ava, the Alg/Ava scaffold effectively diminishes both the extent and strength of adhesion bands. Histopathological examination shows that the reduction in fibrosis and inflammation is responsible for preventing adhesion bands by the Alg/Ava scaffold. Additionally, the cytokine assessment reveals that this is due to the inhibition in the secretion of VEGF and Interleukin 6 suppressing vascularization and inflammatory pathways. This study suggests that a 3D-printed Alg/Ava scaffold has great potential to prevent the postsurgical adhesion bands.
AB - Postoperative adhesion can cause complications, such as pain and organ blockage, in the abdominal regions. To address this issue, surgical techniques and antiadhesive treatments are applied. Given the significant role of vascularization in adhesion band formation, Avastin (Ava) that targets vascular endothelial growth factor (VEGF) can be applied to prevent peritoneal adhesion bands. Moreover, Alginate (Alg), a natural polysaccharide, is a promising physical barrier to prevent adhesion bands. Incorporating Ava into Alg hydrogel in a form of 3D-printed scaffold (Alg/Ava) has potential to suppress inflammation and angiogenesis, leading to reduce peritoneal adhesion bands. Following physical, morphological, and biocompatibility evaluations, the efficacy of Alg and Ava alone and their combination in Alg/Ava on the formation of postsurgical adhesions is evaluated. Upon confirming physical stability and sustained release of Ava, the Alg/Ava scaffold effectively diminishes both the extent and strength of adhesion bands. Histopathological examination shows that the reduction in fibrosis and inflammation is responsible for preventing adhesion bands by the Alg/Ava scaffold. Additionally, the cytokine assessment reveals that this is due to the inhibition in the secretion of VEGF and Interleukin 6 suppressing vascularization and inflammatory pathways. This study suggests that a 3D-printed Alg/Ava scaffold has great potential to prevent the postsurgical adhesion bands.
KW - 3D-printing
KW - adhesion bands
KW - alginate
KW - avastin
KW - fibrosis
KW - inflammation
KW - vascularization
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UR - http://www.scopus.com/inward/citedby.url?scp=85184713988&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/31007856-f1df-3ee7-bd08-57e83ad456d4/
U2 - 10.1002/mabi.202300530
DO - 10.1002/mabi.202300530
M3 - Article
C2 - 38319279
AN - SCOPUS:85184713988
SN - 1616-5187
JO - Macromolecular Bioscience
JF - Macromolecular Bioscience
ER -