Artificial microRNA-mediated resistance against Oman strain of tomato yellow leaf curl virus

Maha R. Al-Roshdi, Ume Ammara, Jamal Khan, Abdullah M. Al-Sadi, Muhammad Shafiq Shahid*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Tomato yellow leaf curl virus (TYLCV) is a global spreading begomovirus that is exerting a major restraint on global tomato production. In this transgenic approach, an RNA interference (RNAi)-based construct consisting of sequences of an artificial microRNA (amiRNA), a group of small RNA molecules necessary for plant cell development, signal transduction, and stimulus to biotic and abiotic disease was engineered targeting the AC1/Rep gene of the Oman strain of TYLCV-OM. The Rep-amiRNA constructs presented an effective approach in regulating the expression of the Rep gene against TYLCV as a silencing target to create transgenic Solanum lycopersicum L. plant tolerance against TYLCV infection. Molecular diagnosis by PCR followed by a Southern hybridization analysis were performed to confirm the effectiveness of agrobacterium-mediated transformation in T0/T1-transformed plants. A substantial decrease in virus replication was observed when T1 transgenic tomato plants were challenged with the TYLCV-OM infectious construct. Although natural resistance options against TYLCV infection are not accessible, the current study proposes that genetically transformed tomato plants expressing amiRNA could be a potential approach for engineering tolerance in plants against TYLCV infection and conceivably for the inhibition of viral diseases against different strains of whitefly-transmitted begomoviruses in Oman.

Original languageEnglish
Article number1164921
JournalFrontiers in Plant Science
Publication statusPublished - 2023


  • agrobacterium-infiltration
  • artificial microRNA
  • gene silencing
  • RNA interference
  • southern blotting

ASJC Scopus subject areas

  • Plant Science

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