Antimicrobial peptides from the skin secretions of the South-East Asian frog Hylarana erythraea (Ranidae)

Nadia Al-Ghaferi, Jolanta Kolodziejek, Norbert Nowotny, Laurent Coquet, Thierry Jouenne, Jérôme Leprince, Hubert Vaudry, Jay D. King, J. Michael Conlon*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)


Peptidomic analysis of norepinephrine-stimulated skin secretions of the South-East Asian frog Hylarana erythraea (formerly Rana erythraea partim) has led to the identification of multiple peptides with antimicrobial activity. Structural characterization of the peptides demonstrated that they belong to the brevinin-1 (3), brevinin-2 (2), esculentin-2 (4), and temporin (1) families. The values in parentheses indicate the number of paralogs. In addition, a peptide (GVIKSVLKGVAKTVALG ML.NH2) was isolated that shows some structural similarity to the brevinin-2-related peptides (B2RP) previously isolated from North American frogs of the genus Lithobates. A synthetic replicate of the species B2RP showed broad-spectrum growth inhibitory activity against reference strains of Escherichia coli (MIC = 12.5 μM), Staphylococcus aureus (MIC = 12.5 μM) and Candida albicans (MIC = 50 μM) and was active against multidrug-resistant clinical isolates of Acetinobacter baumannii (MIC in the range 6-12.5 μM). The hemolytic activity of the peptide was relatively low (LC50 = 280 μM). Phylogenetic analysis based upon the amino acid sequences of 47 brevinin-2 peptides from 17 Asian species belonging to the family Ranidae provides support for the placement of H. erythraea in the genus Hylarana.

Original languageEnglish
Pages (from-to)548-554
Number of pages7
Issue number4
Publication statusPublished - Apr 2010
Externally publishedYes


  • Antimicrobial peptide
  • Brevinin
  • Esculentin-2
  • Frog skin
  • Hylarana
  • Temporin

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience


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