MalforminA1 induce cytotoxicity by targeting Cytoskeleton-related genes in ovarian cancer (OC) cell lines

Epithelial Ovarian cancer (EOC) is a lethal gynecological malignancy characterized by high mortality rates due to the absence of reliable biomarkers, recurrence, and drug resistance. Despite significant progress in cancer research, chemotherapy resistance remains a major concern in OC treatment. In a previous study, we demonstrated that MalforminA1 (MA1), a compound derived from marine sources, exhibited high toxicity toward cisplatin-sensitive (A2780S) and cisplatin-resistant (A2780CP) OC cell lines. The results suggested that MA1 might not activate the canonical apoptosis pathway in EOC cells. However, the exact mechanism of MA1 induced cell death is still elusive. Therefore, in this study we will investigate the impact of MA1 on cell morphology, motility, and intracellular organization. We will utilize western blotting and immunofluorescence techniques to quantify the expression of ?-Tubulin, ?-Tubulin, FAT4, Vimentin, ?-actin, GAPDH, ?-catenin, E-cadherin, and N-cadherin. Our study will shed light on the novel mechanism of MA1 induced cell death by analyzing the alterations in the expression of key cytoskeleton related genes, thereby unravelling the changes in the intracellular organization. Furthermore, the aberrantly expressed cytoskeleton related proteins thus revealed could be utilized as potential targets for cancer treatment.