TY - JOUR
T1 - UV-visile absorption and fluorescence characteristics of the luminescent label coumarin-6-sulphonyl chloride in homogeneous and micellar solutions
AU - Al-Kindy, S. M.Z.
AU - El-Sherbini, S. A.
AU - Abdel-Kader, M. H.
PY - 1994/1/28
Y1 - 1994/1/28
N2 - This paper describes the study of the UV-visual absorption, Fluorescence emission, and fluorescence excitation spectra of coumarin-6-sulphonyl chloride (C-6SCl) in various organic solvents and buffer solutions. In addition, the spectral behaviour in aqueous micellar systems of anionic and cationic surfactant was investigated. The spectral properties of the compound together with the detemined pK values in the ground and excited state are discussed in relation to its electronic structure. The results reflect the importance of the medium effect on the analytical application of C-6SCl as a potential luminescent label for derivatization of amino and phenolic groups.
AB - This paper describes the study of the UV-visual absorption, Fluorescence emission, and fluorescence excitation spectra of coumarin-6-sulphonyl chloride (C-6SCl) in various organic solvents and buffer solutions. In addition, the spectral behaviour in aqueous micellar systems of anionic and cationic surfactant was investigated. The spectral properties of the compound together with the detemined pK values in the ground and excited state are discussed in relation to its electronic structure. The results reflect the importance of the medium effect on the analytical application of C-6SCl as a potential luminescent label for derivatization of amino and phenolic groups.
KW - Coumarin-6-sulphonyl chloride
KW - Luminescence
KW - Micellar solutions
KW - UV-Visible spectrophotometry
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U2 - 10.1016/0003-2670(94)80072-3
DO - 10.1016/0003-2670(94)80072-3
M3 - Article
AN - SCOPUS:0028012510
SN - 0003-2670
VL - 285
SP - 329
EP - 333
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
IS - 3
ER -