TY - JOUR
T1 - The liver flukes Fasciola gigantica and Fasciola hepatica express the leucocyte cluster of differentiation marker CD77 (globotriaosylceramide) in their tegument
AU - Wuhrer, M.
AU - Berkefeld, C.
AU - Dennis, R. D.
AU - Idris, M. A.
AU - Geyer, R.
N1 - Funding Information:
We wish to acknowledge the expert technical assistance of Peter Käse, Werner Mink and Siegfried Kühnhardt. We thank Ivonne Stamm and Dr. Christian Menge, Institut für Hygiene und Infektionskrankheiten der Tiere, Universität Giessen, for experimental help and the kind gift of StxB1 and mAb 13C4. We are grateful to Dr. Tarig Dafa’alla, Institut für Parasitologie, Univer-sität Giessen, for his help with the immunohistochemistry and Dr. Ibtisam Ghoraish, Veterinary Research Centre, Soba, Sudan, for the kind gift of bovine infection sera. This study was supported by the Deutsche Forschungsgemeinschaft (SFB 535, Teilpro-jekte A8 und Z1).
PY - 2001
Y1 - 2001
N2 - Glycosphingolipids from the parasitic liver flukes Fasciola gigantica and Fasciola hepatica were isolated and their carbohydrate moieties were structurally analysed by methylation analysis, exoglycosidase treatment, on-target exoglycosidase cleavage and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. For both liver fluke species, the ceramide monohexosides Gal1-ceramide and Glc1-ceramide were found in relative amounts of 1.0 to 0.1, respectively. From F. gigantica, the ceramide dihexoside was isolated in sufficient amounts to be structurally determined as lactosylceramide, Galβ4-Glc1-ceramide, while for both liver fluke species the ceramide trihexoside was shown to be Galα4Galβ4-Glc1-ceramide, which is designated as either globotriaosylceramide, Pk-blood group antigen or CD77 leucocyte cluster of differentiation antigen. To our knowledge, this is the first report on the expression of globo-series glycosphingolipids in non-mammalian species. Ceramide analysis of ceramide monohexosides yielded as major components octadecanoic and 2-hydroxyoctadecanoic fatty acids together with C18-and C20-phytosphingosines. By the use of an anti-CD77 monoclonal antibody and the Escherichia coli Shiga toxin B1 subunit, globotriaosylceramide could be immunolocalised to the tegument of F. hepatica cryosections. The sharing of CD77 between liver flukes and their mammalian hosts fits in with the concept of molecular mimicry, which is closely parallel to the established imitation of host CD15 (Lewis X) displayed by the blood fluke Schistosoma mansoni.
AB - Glycosphingolipids from the parasitic liver flukes Fasciola gigantica and Fasciola hepatica were isolated and their carbohydrate moieties were structurally analysed by methylation analysis, exoglycosidase treatment, on-target exoglycosidase cleavage and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. For both liver fluke species, the ceramide monohexosides Gal1-ceramide and Glc1-ceramide were found in relative amounts of 1.0 to 0.1, respectively. From F. gigantica, the ceramide dihexoside was isolated in sufficient amounts to be structurally determined as lactosylceramide, Galβ4-Glc1-ceramide, while for both liver fluke species the ceramide trihexoside was shown to be Galα4Galβ4-Glc1-ceramide, which is designated as either globotriaosylceramide, Pk-blood group antigen or CD77 leucocyte cluster of differentiation antigen. To our knowledge, this is the first report on the expression of globo-series glycosphingolipids in non-mammalian species. Ceramide analysis of ceramide monohexosides yielded as major components octadecanoic and 2-hydroxyoctadecanoic fatty acids together with C18-and C20-phytosphingosines. By the use of an anti-CD77 monoclonal antibody and the Escherichia coli Shiga toxin B1 subunit, globotriaosylceramide could be immunolocalised to the tegument of F. hepatica cryosections. The sharing of CD77 between liver flukes and their mammalian hosts fits in with the concept of molecular mimicry, which is closely parallel to the established imitation of host CD15 (Lewis X) displayed by the blood fluke Schistosoma mansoni.
KW - Liver fluke glycolipids
KW - Oligosaccharide structural analysis
KW - On-target enzymatic cleavage
KW - Trematode
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U2 - 10.1515/BC.2001.027
DO - 10.1515/BC.2001.027
M3 - Article
C2 - 11308018
AN - SCOPUS:0035086666
SN - 1431-6730
VL - 382
SP - 195
EP - 207
JO - Biological Chemistry
JF - Biological Chemistry
IS - 2
ER -