TY - JOUR
T1 - Real-time quantitative PCR assay for the quantification of virus and satellites causing leaf curl disease in cotton in Pakistan
AU - Shafiq, Muhammad
AU - Iqbal, Zafar
AU - Ali, Irfan
AU - Abbas, Qamar
AU - Mansoor, Shahid
AU - Briddon, Rob W.
AU - Amin, Imran
N1 - Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/10
Y1 - 2017/10
N2 - Cotton leaf curl disease (CLCuD) is the major biotic constraint to cotton production in Pakistan and northwestern India. The disease is caused by monopartite begomoviruses in association with a specific DNA satellite, Cotton leaf curl Multan betasatellite. The virus-betasatellite complex is also frequently associated with another DNA satellite-like molecule; an alphasatellite. A quantitative real-time PCR (qPCR) assay to detect all three components of the monopartite begomovirus/betasatellite/alphasatellite complex which causes CLCuD was established. This was used to investigate the relationship between symptoms and virus/satellite titre. Not surprisingly the analysis showed that, overall, there was a reasonable correlation between symptom severity and virus/satellite titre − more severe symptoms usually being associated with more virus/satellite. However, cotton plants were identified with no or very mild symptoms with relatively high virus/satellite titres and plants with severe symptoms but relatively low virus/satellite titres. This may be attributed to the resistance/susceptibility of the cotton variety − tolerant plants being able to sustain a relatively high virus/satellite titre whilst exhibiting mild symptoms. The usefulness of this qPCR procedure in the screening for resistance in cotton against CLCuD is discussed.
AB - Cotton leaf curl disease (CLCuD) is the major biotic constraint to cotton production in Pakistan and northwestern India. The disease is caused by monopartite begomoviruses in association with a specific DNA satellite, Cotton leaf curl Multan betasatellite. The virus-betasatellite complex is also frequently associated with another DNA satellite-like molecule; an alphasatellite. A quantitative real-time PCR (qPCR) assay to detect all three components of the monopartite begomovirus/betasatellite/alphasatellite complex which causes CLCuD was established. This was used to investigate the relationship between symptoms and virus/satellite titre. Not surprisingly the analysis showed that, overall, there was a reasonable correlation between symptom severity and virus/satellite titre − more severe symptoms usually being associated with more virus/satellite. However, cotton plants were identified with no or very mild symptoms with relatively high virus/satellite titres and plants with severe symptoms but relatively low virus/satellite titres. This may be attributed to the resistance/susceptibility of the cotton variety − tolerant plants being able to sustain a relatively high virus/satellite titre whilst exhibiting mild symptoms. The usefulness of this qPCR procedure in the screening for resistance in cotton against CLCuD is discussed.
KW - Cotton leaf curl Khokhran virus − Burewala (CLCuKoV-Bu)
KW - Cotton leaf curl Multan betasatellite (CLCuMuB)
KW - Cotton leaf curl disease (CLCuD)
KW - Polymerase chain reaction (PCR)
KW - Quantitative real-time PCR (qPCR)
UR - http://www.scopus.com/inward/record.url?scp=85021065546&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85021065546&partnerID=8YFLogxK
U2 - 10.1016/j.jviromet.2017.05.012
DO - 10.1016/j.jviromet.2017.05.012
M3 - Article
C2 - 28572041
AN - SCOPUS:85021065546
SN - 0166-0934
VL - 248
SP - 54
EP - 60
JO - Journal of Virological Methods
JF - Journal of Virological Methods
ER -