TY - JOUR
T1 - Protein interaction mapping reveals widespread targeting of development-related host transcription factors by phytoplasma effectors
AU - Correa Marrero, Miguel
AU - Capdevielle, Sylvain
AU - Huang, Weijie
AU - Al-Subhi, Ali M.
AU - Busscher, Marco
AU - Busscher-Lange, Jacqueline
AU - van der Wal, Froukje
AU - de Ridder, Dick
AU - van Dijk, Aalt D.J.
AU - Hogenhout, Saskia A.
AU - Immink, Richard G.H.
N1 - Publisher Copyright:
© 2023 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.
PY - 2023
Y1 - 2023
N2 - Phytoplasmas are pathogenic bacteria that reprogram plant host development for their own benefit. Previous studies have characterized a few different phytoplasma effector proteins that destabilize specific plant transcription factors. However, these are only a small fraction of the potential effectors used by phytoplasmas; therefore, the molecular mechanisms through which phytoplasmas modulate their hosts require further investigation. To obtain further insights into the phytoplasma infection mechanisms, we generated a protein–protein interaction network between a broad set of phytoplasma effectors and a large, unbiased collection of Arabidopsis thaliana transcription factors and transcriptional regulators. We found widespread, but specific, interactions between phytoplasma effectors and host transcription factors, especially those related to host developmental processes. In particular, many unrelated effectors target specific sets of TCP transcription factors, which regulate plant development and immunity. Comparison with other host-pathogen protein interaction networks shows that phytoplasma effectors have unusual targets, indicating that phytoplasmas have evolved a unique and unusual infection strategy. This study contributes a rich and solid data source that guides further investigations of the functions of individual effectors, as demonstrated for some herein. Moreover, the dataset provides insights into the underlying molecular mechanisms of phytoplasma infection.
AB - Phytoplasmas are pathogenic bacteria that reprogram plant host development for their own benefit. Previous studies have characterized a few different phytoplasma effector proteins that destabilize specific plant transcription factors. However, these are only a small fraction of the potential effectors used by phytoplasmas; therefore, the molecular mechanisms through which phytoplasmas modulate their hosts require further investigation. To obtain further insights into the phytoplasma infection mechanisms, we generated a protein–protein interaction network between a broad set of phytoplasma effectors and a large, unbiased collection of Arabidopsis thaliana transcription factors and transcriptional regulators. We found widespread, but specific, interactions between phytoplasma effectors and host transcription factors, especially those related to host developmental processes. In particular, many unrelated effectors target specific sets of TCP transcription factors, which regulate plant development and immunity. Comparison with other host-pathogen protein interaction networks shows that phytoplasma effectors have unusual targets, indicating that phytoplasmas have evolved a unique and unusual infection strategy. This study contributes a rich and solid data source that guides further investigations of the functions of individual effectors, as demonstrated for some herein. Moreover, the dataset provides insights into the underlying molecular mechanisms of phytoplasma infection.
KW - Arabidopsis
KW - developmental reprogramming
KW - effectors
KW - phytoplasmas
KW - protein-protein interaction
KW - transcription factors
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U2 - 10.1111/tpj.16546
DO - 10.1111/tpj.16546
M3 - Article
C2 - 37965720
AN - SCOPUS:85176932023
SN - 0960-7412
JO - Plant Journal
JF - Plant Journal
ER -