Background: This study aimed to explore the prevalence rates of Hospital Acquired Blood Stream Infections (HA-BSIs) by patients and isolates during the defined period in a tertiary care hospital, Sultan Qaboos University Hospital (SQUH), in Oman. Methods & Materials: Cross-sectional study during the period from January 2013 to December 2013. Data about patients admitted at the tertiary hospital were retrospectively collected during the study period. The cumulative frequency and prevalence rates of HA-BSIs by patients and isolates were calculated. In addition,cumulative frequency of participant with single versus mixed infections and types of causative micro-organisms of HA-BSIs were obtained. Results: Among the 30,764 patients analyzed, HA-BSIs occurred in 268 patients, revealing a prevalence rate of 8.7 patients per 1000 admissions. The majority of patients with HA-BSIs had monomicrobial infection. The most common isolates were coagulase-negative staphylococcus (21.4%), klebsiella pneuminae (12.3%), and candida species (9.4%). Conclusion: This study provides a benchmark for the epidemiology of HA-BSIs in Oman. This rate is lower than rates reported by most developing countries. Further reinforcement of infection prevention and control measures are necessary to reduce these rates further.