TY - JOUR
T1 - Preparation and validated analysis of anthocyanin concentrate from the calyces of Hibiscus sabdariffa
AU - Opletal, Lubomir
AU - Chocholousova-Havlikova, Lucie
AU - Siatka, Tomas
AU - Cahliková, Lucie
AU - Locarek, Miroslav
AU - Ali, Badreldin H.
AU - Manoj, Priyadarshini
AU - Ramkumar, Aishwarya
AU - Al Suleimani, Yousuf M.
AU - Al Za'Abi, Mohammed
AU - Karaca, Turan
AU - Nemmar, Abderrahim
PY - 2017/1
Y1 - 2017/1
N2 - Extracts of Hibiscus sabdariffa calyces have been shown to have various medicinal properties, some of which have been reported to be due to anthocyanins present in the calyces. To study whether these claims are valid, it is necessary to produce an extract with a high anthocyanin content and to have available a method to identify and quantify the individual compounds in the product. A method of extraction and purification has been developed based on a polyamide column chromatographic purification step. Using this method, anthocyanin concentrates were produced containing from 57 to 64% of delphinidin-3-sambubioside plus cyanidin-3-sambubioside. A rapid, efficient and validated HPLC analytical method was developed and used for the analysis of the anthocyanin concentrate.
AB - Extracts of Hibiscus sabdariffa calyces have been shown to have various medicinal properties, some of which have been reported to be due to anthocyanins present in the calyces. To study whether these claims are valid, it is necessary to produce an extract with a high anthocyanin content and to have available a method to identify and quantify the individual compounds in the product. A method of extraction and purification has been developed based on a polyamide column chromatographic purification step. Using this method, anthocyanin concentrates were produced containing from 57 to 64% of delphinidin-3-sambubioside plus cyanidin-3-sambubioside. A rapid, efficient and validated HPLC analytical method was developed and used for the analysis of the anthocyanin concentrate.
KW - Anthocyanins
KW - Calyces
KW - Hibiscus sabdariffa
KW - Malvaceae
KW - Polyamide purification
KW - Validated HPLC analysis.
UR - http://www.scopus.com/inward/record.url?scp=85019107999&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85019107999&partnerID=8YFLogxK
U2 - 10.1177/1934578x1701200112
DO - 10.1177/1934578x1701200112
M3 - Article
AN - SCOPUS:85019107999
SN - 1934-578X
VL - 12
SP - 44
EP - 45
JO - Natural Product Communications
JF - Natural Product Communications
IS - 1
ER -