TY - JOUR
T1 - Induction of pathogenesis-related proteins, phenolics and phenylalanine ammonia-lyase in groundnut by Pseudomonas fluorescens
AU - Meena, B.
AU - Radhajeyalakshmi, R.
AU - Marimuthu, T.
AU - Vidhyasekaran, P.
AU - Doraiswamy, S.
AU - Velazhahan, R.
PY - 2000
Y1 - 2000
N2 - Foliar application of a plant growth-promoting rhizobacterium, Pseudomonas fluorescens strain Pf1 significantly controlled late leaf spot and rust diseases of groundnut (Arachis hypogaea L.) under greenhouse conditions. Groundnut plants, when sprayed with P. fluorescensstrain Pf1, showed increase in activity of phenylalanine ammonia-lyase 1 day after application and the maximum enzyme activity was detected at 3 days after treatment. An increase in phenolic content was observed 1 day after P. fluorescens treatment. Also chitinase and β-1,3-glucanase activities increased significantly 1 day after P. fluorescens treatment and maximum activities were reached 4 days later. Application of P. fluorescens to groundnut leaves induced accumulation of a 23-kDa thaumatin-like protein (TLP) and a 30-kDa glucanase. The induction of 23-kDa TLP was detected only in treated leaves and not in roots, stems and petioles of the groundnut plants. The 30-kDa glucanase started to accumulate 1 day after treatment and the level of the protein continued to increase throughout the experimental period of 4 days. Both TLP and glucanase were found in the intercellular fluid of P. fluorescens-sprayed groundnut leaves.
AB - Foliar application of a plant growth-promoting rhizobacterium, Pseudomonas fluorescens strain Pf1 significantly controlled late leaf spot and rust diseases of groundnut (Arachis hypogaea L.) under greenhouse conditions. Groundnut plants, when sprayed with P. fluorescensstrain Pf1, showed increase in activity of phenylalanine ammonia-lyase 1 day after application and the maximum enzyme activity was detected at 3 days after treatment. An increase in phenolic content was observed 1 day after P. fluorescens treatment. Also chitinase and β-1,3-glucanase activities increased significantly 1 day after P. fluorescens treatment and maximum activities were reached 4 days later. Application of P. fluorescens to groundnut leaves induced accumulation of a 23-kDa thaumatin-like protein (TLP) and a 30-kDa glucanase. The induction of 23-kDa TLP was detected only in treated leaves and not in roots, stems and petioles of the groundnut plants. The 30-kDa glucanase started to accumulate 1 day after treatment and the level of the protein continued to increase throughout the experimental period of 4 days. Both TLP and glucanase were found in the intercellular fluid of P. fluorescens-sprayed groundnut leaves.
KW - Chitinase
KW - Groundnut
KW - Phenolics
KW - Phenylalanine ammonia-lyase
KW - Pseudomonas fluorescens
KW - Thaumatin-like protein
KW - β-1,3-glucanase
UR - http://www.scopus.com/inward/record.url?scp=0033762131&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033762131&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0033762131
SN - 0340-8159
VL - 107
SP - 514
EP - 527
JO - Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz
JF - Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz
IS - 5
ER -