TY - JOUR
T1 - Genotypic analysis of hepatitis E virus (HEV) from sporadic symptomatic cases in Pakistan
AU - Ejaz, Momina
AU - Zohaib, Ali
AU - Usman, Muhammad
AU - Anwar, Muhammad Moaaz
AU - Khan, Hafiz Sajid
AU - Ammar, Muhammad
AU - Saqib, Muhammad
AU - Khan, Sadia Ghani
AU - Athar, Muhammad Ammar
AU - Mansoor, Muhammad Khalid
AU - Ullah, Ahsaan
AU - Naseem, Mehvish
AU - Hussain, Muhammad Hammad
AU - Javed, Aneela
N1 - Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.
PY - 2024/3/1
Y1 - 2024/3/1
N2 - Pakistan is the fifth most populous nation in the world and faces several challenges, including devastating floods, sub-optimal sanitary conditions, clustered accommodations, and unregulated cross-border movements. These drastic population shifts make it vulnerable to the efficient spread of the Hepatitis E virus (HEV). The current study analyzed the genotypic characteristics and variants of the Hepatitis E virus circulating in the population of Pakistan. A total of 75 ELISA-IgM positive samples were collected from three metropolitan cities: Lahore, Peshawar, and Karachi, and subjected to viral RNA extraction. The amplification of the HEV RNA-dependent RNA polymerase (RdRp) region was done using Nested PCR and degenerate primers. Out of the total, 40% of the samples were positive for HEV RNA. Sequencing and phylogenetic analysis identified the new HEV isolates as Subtype 1 g, a subtype within an existing HEV genotype 1. This shift warrants investigation into its impact on clinical manifestation and disease severity. Importantly, this study marks the first HEV subtype analysis in Pakistan, contributing valuable insights into subtype diversity and prevalence in the region.
AB - Pakistan is the fifth most populous nation in the world and faces several challenges, including devastating floods, sub-optimal sanitary conditions, clustered accommodations, and unregulated cross-border movements. These drastic population shifts make it vulnerable to the efficient spread of the Hepatitis E virus (HEV). The current study analyzed the genotypic characteristics and variants of the Hepatitis E virus circulating in the population of Pakistan. A total of 75 ELISA-IgM positive samples were collected from three metropolitan cities: Lahore, Peshawar, and Karachi, and subjected to viral RNA extraction. The amplification of the HEV RNA-dependent RNA polymerase (RdRp) region was done using Nested PCR and degenerate primers. Out of the total, 40% of the samples were positive for HEV RNA. Sequencing and phylogenetic analysis identified the new HEV isolates as Subtype 1 g, a subtype within an existing HEV genotype 1. This shift warrants investigation into its impact on clinical manifestation and disease severity. Importantly, this study marks the first HEV subtype analysis in Pakistan, contributing valuable insights into subtype diversity and prevalence in the region.
KW - Hepatitis E virus
KW - HEV subtype 1 g
KW - Low- and middle-income countries
KW - Water-borne outbreaks
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UR - https://www.mendeley.com/catalogue/781de97a-0edf-3730-83c8-a2be7d6596e4/
U2 - 10.1016/j.meegid.2024.105567
DO - 10.1016/j.meegid.2024.105567
M3 - Article
C2 - 38342162
SN - 1567-1348
VL - 118
SP - 105567
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
M1 - 105567
ER -