TY - JOUR
T1 - Genome-Wide Analysis and Expression Profiling of DUF668 Genes in Glycine max under Salt Stress
AU - Zaynab, Madiha
AU - Sharif, Yasir
AU - Xu, Zhaoshi
AU - Fiaz, Sajid
AU - Al-Yahyai, Rashid
AU - Yadikar, Hamad A.
AU - Al Kashgry, Najla Amin T.
AU - Qari, Sameer H.
AU - Sadder, Monther
AU - Li, Shuangfei
N1 - Publisher Copyright:
© 2023 by the authors.
PY - 2023/8/11
Y1 - 2023/8/11
N2 - The DUF668 gene performs a critical role in mitigating the impact of abiotic stress factors. In this study, we identified 30 DUF668 genes in a soybean genome, distributed across fifteen chromosomes. The phylogenetic analysis classified the DUF668 genes into three groups (group I, group II, and group III). Interestingly, gene structure analysis illustrated that several GmDUF668 genes were without introns. Furthermore, the subcellular localization results suggested that GmDUF668 proteins were present in the nucleus, mitochondria, cytoplasm, and plasma membrane. GmDUF668 promoters were analyzed in silico to gain insight into the presence of regulatory sequences for TFs binding. The expression profiling illustrated that GmDUF668 genes showed expression in leaves, roots, nodules, and flowers. To investigate their response to salt stress, we utilized the RNA sequencing data of GmDUF668 genes. The results unveiled that GmDUF668-8, GmDUF668-20, and GmDUF668-30 genes were upregulated against salt stress treatment. We further validated these findings using qRT-PCR analysis. These findings provide a scientific basis to explore the functions of GmDUF668 genes against different stress conditions.
AB - The DUF668 gene performs a critical role in mitigating the impact of abiotic stress factors. In this study, we identified 30 DUF668 genes in a soybean genome, distributed across fifteen chromosomes. The phylogenetic analysis classified the DUF668 genes into three groups (group I, group II, and group III). Interestingly, gene structure analysis illustrated that several GmDUF668 genes were without introns. Furthermore, the subcellular localization results suggested that GmDUF668 proteins were present in the nucleus, mitochondria, cytoplasm, and plasma membrane. GmDUF668 promoters were analyzed in silico to gain insight into the presence of regulatory sequences for TFs binding. The expression profiling illustrated that GmDUF668 genes showed expression in leaves, roots, nodules, and flowers. To investigate their response to salt stress, we utilized the RNA sequencing data of GmDUF668 genes. The results unveiled that GmDUF668-8, GmDUF668-20, and GmDUF668-30 genes were upregulated against salt stress treatment. We further validated these findings using qRT-PCR analysis. These findings provide a scientific basis to explore the functions of GmDUF668 genes against different stress conditions.
KW - abiotic stress
KW - duplication
KW - gene expression
KW - miRNAs
KW - phylogeny
KW - phytohormones
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UR - https://www.mendeley.com/catalogue/06bf7da4-b015-3e03-8391-80403f539331/
U2 - 10.3390/plants12162923
DO - 10.3390/plants12162923
M3 - Article
C2 - 37631135
AN - SCOPUS:85169133907
SN - 2223-7747
VL - 12
JO - Plants
JF - Plants
IS - 16
M1 - 2923
ER -